RESUMEN
ABSTRACT NKX3.1 and PTEN genes are involved in the development and progression of prostate cancer (PCa). Here, in line with other studies that correlated the expression of these two genes, we aimed at evaluating the expression pattern of these genes in clinical PCa samples. Collectively, 81 tissue samples including 45 human PCa and 36 benign prostatic hyperplasia (BPH) specimens were included in the study. The tissue samples were subjected to RNA extraction and subsequently to cDNA synthesis according to the kit manufacturer's protocol. Quantitative Real-Time PCR assay was performed for each sample in triplicate reactions. REST and SPSS software were used to statistically analyze PTEN and NKX3.1 gene expression data. Expression level of both NKX3.1 and PTEN genes was down-regulated in PCa samples compared to BPH samples. The relative expression ratio of PTEN and NKX3.1 was decreased to 0.155 and 0.003, respectively (P=0.000). The results of Chi-Square analysis revealed a significant correlation between the expression of these genes in both BPH and cancer groups (P=0.004 and 0.001, respectively). According to previous studies and our data, we concluded that the association between the down-regulation of PTEN and NKX3.1 genes contributed to the prostate tumorigenesis. This might highlight the interaction between the proteins encoded by these genes. Furthermore, this finding might be exploited for the development of innovative diagnostic and therapeutic approaches in PCa.
Asunto(s)
Anciano , Anciano de 80 o más Años , Humanos , Masculino , Persona de Mediana Edad , Regulación hacia Abajo , Expresión Génica , Proteínas de Homeodominio/genética , Fosfohidrolasa PTEN/genética , Neoplasias de la Próstata/genética , Factores de Transcripción/genética , Carcinogénesis/genética , Progresión de la Enfermedad , Electroforesis en Gel Bidimensional , Marcadores Genéticos , Proteínas de Homeodominio/análisis , Fosfohidrolasa PTEN/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Valores de Referencia , Temperatura , Temperatura de Transición , Factores de Transcripción/análisisRESUMEN
Aim of this study was to assess the association between prostate calcification in transrectalultrasound [TRUS] and sonoguided needle biopsy in prostate cancer [PCa]. A cross-sectional study was performed on patients referred to Imam Khomeini Hospital between 2005 and 2011 who underwent TRUS biopsy due to increased PSA or abnormal DRE. The serum levels of PSA, prostate volume and transition zone volume and Gleason grade were compared between patients with and without calcification. A total of 1608 cases were included and the mean age of patients was 66.3 +/- 8.6 [range: 37-96] years. Calcification was observed in 105 cases [6.5%] and 515 [32%] hadPCa. There was no statistically significant relationship between PCa and calcification on TRUS. Among non-cancerous patients, there was a significant difference in the mean PSA level among individuals with and without calcification PSA [10.3 +/- 7.8 vs. 8.9 +/- 7.6; P=0.028]. There was no difference between subjects with and without calcification in mean prostate volume and transition lobe volume among all patients and also Gleason score in patients with PCa. Our study is the first in Iran that evaluate the relationship between calcification in ultrasound and PCa. The incidence of calcification in TRUS among Iranian patients was 6.5%. It is showed that there is no statistically significant association between prostate cancer and existence of prostate tissue calcification in TRUS