Extended spectrum beta-lactamase-producing bacteria among diabetic patients with asymptomatic bacteriuria in a secondary health facility in Edo State, Nigeria

Asymptomatic bacteriuria (ASB) can increase the risk for developing symptomatic urinary tract infection (UTI) and diabetes mellitus is one of the high risk medical condition for ASB. This study was carried out to determine the prevalence of extended spectrum beta-lactamase (ESBL)-producing bacteria in diabetic patients attending Central Hospital, Benin City. The descriptive cross-sectional study design was adopted for this study, while data was obtained with the use of a structured questionnaire administered on 695 consented diabetic patients. Statistical analysis was done using the statistical software INSTAT� (Graph Pad Software Inc., La Jolla, CA, USA). Mid-stream urine samples were aseptically collected from these patients, processed and analysed using standard techniques. Identification of bacterial isolates and antimicrobial susceptibility testing were carried out using standard microbiological techniques. ESBL-producers were detected using appropriate phenotypic method. A total of 253(36.4%) patients were culture positive with Escherichia coli being the most predominant organism (37.5%). Proteus mirabilis was the highest ESBL producer and was statistically significant (50.0%) (p=0.0095). Uncontrolled glycaemia was associated with ESBL-production among bacterial isolates (P=0.0129). Age, gender and source of clinical isolates did not significantly affect ESBL production by the isolated uropathogenic bacteria (P >0.05).The prevalence of ESBL-producing bacteria among diabetic patients having ASB was 36.8%. Adherence to proper and effective antimicrobial regimen and laboratory guidance in treatment of ASB among diabetic patients are recommended

Prevalence of Anaemia among HIV-Infected patients in Benin City; Nigeria

The aim of this study was to use the World Health Organization (WHO) definition of anaemia to determine prevalence of anaemia among human immunodeficiency virus (HIV)-infected patients on the highly active antiretroviral therapy (HAART) and those that are HAART naive. Haemoglobin concentration was measured in 457 HIV patients consisting of 217 patients on HAART (86 males and 131 females) and 240 HAART naive patients (106 males and 134 females). According to WHO criteria; anaemia was defined as a haemoglobin concentration below 12g/dl in women and below 13g/dl in men. The anaemic HIV patients were further categorized according to WHO/ACTG anaemia toxicity grades. An overall anaemia prevalence of 60.61was observed. The prevalence of anaemia was significantly higher among HAART naive patients (69.17) than in HIV patients on HAART (51.15) (P 0.001). The prevalence of anaemia differ significantly (P 0.05) between males and females of HAART naive patients with males (76.42) having higher prevalence than females (63.43). The WHO/ACTG categorization showed the same pattern between HIV patients on HAART and those that were HAART naive. Conclusively; the overall prevalence of anaemia was 60.61among HIV patients. HAART naive patients have higher prevalence as well as males in this group. The WHO definition of anaemia is recommended as this will give the true prevalence of anaemia and allow for policy and interventions to address it

Effects of gender and seasonal variation on the prevalence of bacterial septicemia among young children in Benin city, Nigeria

Aim: To determine the effects of gender and seasonal variations on the prevalence of bacterial septicaemia among children 5 years and younger; and to identify the bacterial agents responsible for septicaemia and their antibiotic susceptibility profiles. Methods: Blood was collected from 1;724 children (967 males and 757 females) aged 1 day to 5 years with clinical signs and symptoms of septicaemia. This study was carried out from 1 January to 31 December 2007 at the University of Benin Teaching Hospital; Benin City; Nigeria. The blood samples were processed to diagnose bacterial septicaemia. Bacterial isolates were identified and susceptibility test was performed using standard techniques. Results: An overall prevalence of 22.10of confirmed bacterial septicaemia was observed in this study. Generally; gender and seasonal variations did not significantly affect the prevalence of bacterial septicaemia; though females (50.57) during the dry season had significantly (p 0.001) higher prevalence than their male counterparts (19.91). Staphylococcus aureus was the predominant bacterial isolate causing septicaemia in both seasons; while Citrobacter freundii was the least frequent. Pseudomonas aeruginosa was not recovered during the dry season. Most isolates were susceptible to gentamicin and cefuroxime; but only 1.44of Staphylococcus aureus strains were susceptible to ceftriaxone. Conclusion: Bacterial septicaemia was observed in 22.1of children 5 years and younger with clinical signs and symptoms of septicaemia. Seasonal variation did not affect the prevalence. Effect of gender was only noticed in the dry season; where females had a higher prevalence than males. Gentamicin and cefuroxime were the most active antibacterial agents. Rational use of antibiotics is advocated