Prevalence of Cryptosporidium oocysts in faecal samples of some school children in Enugu State, Nigeria.

A total of 373 stool samples comprising 38 watery stool and 335 normal formed samples, collected from primary school children in Enugu State, Nigeria, were processed for the presence of Cryptosporidium oocysts. The formalin-methylene blue staining techniques were employed for the concentration and identification of the Oocysts in each sample. The results obtained were subjected to cross-tabulation analysis. Cryptosporidium Oocysts were identified in 15 of the 38 watery stool samples and in 81 of the 335 formed stool samples. Analysis of the data obtained showed that there was no significant difference in carrier rate of the parasite between school children from the urban town of Nsukka and those from the rural towns of Ovoko and Obukpa (P = 0.441). However, the rate of occurrence of the Oocysts was significantly higher in the watery stool than in the formed stool samples (P = < 0.0001).

Cryptosporidiosis in patients with diarrhoea in five hospitals in Nigeria.

A total of 413 soft, loose or watery stool specimens from patients with acute diarrhoea were screened for presence of Cryptosporidium oocysts. Safranin-methylene blue staining technique was used for detecting Cryptosporidium oocysts in the stool samples. The oocysts were identified in 52 (12.5 per cent) of the samples, while 69 samples (16.7 per cent) showed mixed infections. Children between the ages of 2 and 15 years were most infected by this parasite while infection occurred more in females than males for all age groups. These findings indicate that Cryptosporidium is an important etiologic agent of diarrhoea. Hence there is need to include its identification in laboratory analysis of diarrhoeal stool samples.