Subconjunctival steroid for pearls like tumour in the anterior chamber of the eye

Pearl like tumor in the anterior chamber of the eye is a not uncommon affection of children eyes, difficult to tract by topical and/or systemic steroid assuming that the condition is allergic in origin. To assess the effect of sub-conjunctival steroid injection in these cases. It is a retrospective study for all patients aged between 10 - 18 years presenting with a unilateral grayish white nodule at the angle of the anterior chamber of the eye sub-conjunctival long acting steroid was given for all patients and the follow up was done for one year. 10 eyes of 10 patients were included in this research. One child was a new case and 9 were recurrent cases. All 10 then had varying degree of anterior ureitis. After steroid injection the severity of iritic decreased in all patients. The size of the lesion decreased to more than 50% after 2 months. sub-conjunctival steroid injection for pearl like lesion in the anterior chamber of the eye is a safe and effective way of treating such lesion

Characterization of toxocara canis excretory secretory antigen and its application for diagnosis of clinically suspected cases of ocular toxocariasis by western blot analysis

Toxocariasis is a frequent heluminthiasis that can cause visceral and ocular damage in humans. The present work represents three cases of suspected ocular toxocariasis [OT]. Complaining of visual impairment. Ocular toxocariasis was suspected based on clinical manifestations. Diagnosis was confirmed by Western blot analysis. The identification of specific antigen of Toxocara canis is important in order to develop better diagnostic techniques. Toxocara excretory secretory antigen [TES] was prepared and it was characterized by SDS-PAGE. A characteristic pattern of nine bands was achieved. These bands were at [17, 26, 32, 35, 45, 55, 70, 90, and 120] KDa. Components of T. canis ES antigen [TES] were identified by sera of clinically suspected cases of ocular loxocariasis [OT] by using [WB] analysis. The identified proteins were grouped into one low molecular weight fraction [26-35 KDa] and one high molecular weight fraction [55-90 KDa]. These antigenic bands could be considered as a guide for the diagnosis of ocular toxocariasis. Systemic albendazole was given conjunction with steroids Treatment resulted in an improved visual acuity in all patients

Immunoblotting and enzyme linked-immunosorbent assay for diagnosis of ocular toxoplasmosis

In the present work, the diagnosis of ocular toxoplasmosis was based on the clinical picture and the serological test results of enzyme linked-immunosorbent assay [IgM-ELISA and IgG-ELISA], ELISA and Western blotting of Toxoplasma antigen against sera from ocular patients in the acute and chronic phases of the disease. Forty-five cases of suspected ocular toxoplasmosis and five control samples were examined clinically and serologically. The clinical diagnosis was based on the manifestation of the characteristic biomicroscopic features, where a white and irregular inflammatory focus was detected frequently in association with an old scar. Fundus examination revealed five cases in the acute phase, twenty-seven cases in the chronic phase and thirteen atypical cases. Sera were collected to be tested serologically by ELISA and Western blot technique, where it was found that the diagnosis was confirmed for 82.3% by ELISA; this rate rose to 88.9% by Western blot technique adding better results. All control sera were negative by ELISA and Western blot. Toxoplasma gondii cyst antigen was prepared, characterized by SDS-PAGE and recognized by antibodies in serum samples from patients presenting with ocular toxoplasmosis by means of Western blot technique. Antigenic bands 22, 23, 24, 25, 27, 66 and 67 kDa could be characterized as antigenic markers for acute ocular toxoplasmosis and 34, 35, 36, 37, 39, 40, 43, 66 and 67 kDa as antigenic markers for chronic ocular toxoplasmosis