Effect of various incubation temperatures on in-Vitro intracellular killing of salmonella typhi

The in vitro intracellular killing of Salmonella typhi by human neutrophils under incubation temperatures of 37°C and 40°C was tested at various time intervals. The study employed bactericidal assay. At 37°C the intracellular killing of the organism was high at the first 30 min. then it became stationary up to 120 min. At 40°C the intracellular killing was observed in the first 20 min. which reversed to active multiplication of the organism inside the neutrophil with further incubations up to 120 min