RESUMEN
ABSTRACT PURPOSE: To investigate the protective effect of β-myrcene (MYR) on oxidative and histological damage in mice heart tissue caused global cerebral ischemia/reperfusion (IR) in C57BL/J6 mice. METHODS: Animals(n=40) were randomly divided into four groups: (1)control, (2)IR, (3)MYR and (4)MYR+IR. The control group was received 0.1% carboxymethyl cellulose as a vehicle following a medial incision without carotid occlusion. In the IR group, the bilateral carotid arteries were clipped for 15min, and treated with the vehicle intraperitoneally(ip) for 10 days. MYR (200mg/kg) was received dissolved in 0.1%CMC for 10 days. In the MYR+IR group, the IR model was applied exactly as in the IR group, and then they were treated with MYR 10 days. RESULTS: The cerebral IR caused oxidative damage (increase TBARS, decrease antioxidant parameters). Treatment of MYR was increased in GSH,GPx,CAT,SOD activity while TBARS level was decreased. In addition, degenerative changes in I/R group heart tissue were ameliorated by MYR administration. CONCLUSİON: The administration of β-myrcene protects oxidative and histological damage in the heart tissue after global ischemia-reperfusion and may be useful safe alternative treatment for cardiac tissue after ischemic stroke.
Asunto(s)
Animales , Masculino , Cardiotónicos/farmacología , Daño por Reperfusión/complicaciones , Isquemia Encefálica/complicaciones , Monoterpenos/farmacología , Corazón/efectos de los fármacos , Antioxidantes/farmacología , Superóxido Dismutasa/metabolismo , Catalasa/metabolismo , Distribución Aleatoria , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Estrés Oxidativo/efectos de los fármacos , Modelos Animales , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Ratones Endogámicos C57BL , Miocardio/metabolismo , Miocardio/patologíaRESUMEN
The blocking of nitric oxide synthase [NOS] activity may reason vasoconstriction with formation of reactive oxygen species. Propolis has biological and pharmacological properties, such as antioxidant. The aim of this study was to examine the antioxidant effects of propolis which natural product on biochemical parameters in brain and lung tissues of acute nitric oxide synthase inhibited rats by Nco-nitro-L-arginine methyl ester [L-NAME]. Rats have been received L-NAME [40 mg/kg, intraperitoneally], NOS inhibitor for 15 days to produce hypertension and propolis [200mg/kg, by gavage] the lastest 5 of 15 days. There were the increase [P0<001] in the malondialdehyde levels in the L-NAME treatment groups when compared to control rats, but the decrease [P<001] in the catalase activities in both brain and lung tissues. There were statistically changes [P<001] in these parameters of L-NAME+propolis treated rats as compared with E-NAME-treated group. The application of L-NAME to the Wistar rats resulted in well developed oxidative stress. Also, propolis may influence endothelial NO production. Identification of such compounds and characterisation of their cellular actions may increase our knowledge of the regulation of endothelial NO production and could provide valuable clues for the prevention or treatment of hypertensive diseases and oxidative stress