[Evaluation of the effects of chlorhexidine 0.12% mouthwash on mouth pathogen streptococcus and normal microflora]

Mouthwashes like brush and dental floss, can cause reduction in dental plaque and gingivitis. An optimal mouthwash should have antimicrobial properties, low drug resistance, and cause no decrease in normal microflora of the mouth. The purpose of this study was the evaluation of the effects of chlorhexidine 0.12% mouthwash on pathogen streptococcus and normal microflora of the mouth. In this experimental study, based on selected inclusion criteria, 28 subjects, were selected and asked to use 0.12% chlorhexidine mouthwash for 2 weeks, according to the manufacturer instruction. Before and after rinsing with the mouthwash, subjects were requested to wash their mouth with physiologic serum. After washing, 1cc of saliva of each individual was collected in sterile tube and immediately sent to microbiology laboratory. This process was repeated 2 weeks after using mouthwash. The number of pathogen streptococcus and normal microflora colonies of the mouth before and after using chlorhexidine were recorded. For analyzing the data, T and Chi Square Test were used.: Chlorhexidine mouthwash [0.12%] significantly decreased numbers of the pathogen streptococcus and mouth normal microflora [p <0/05] This study showed that, 0.12% chlorhexidine mouthwash, can destroy not only the mouth pathogen streptococci, but also the normal microflora of the mouth. The latter should be considered as its side effect

Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR

Brucellosis is one of the most common zoonotic diseases in Iran. Growth of Brucella is slow and blood cultures of these bacteria are time-consuming via classical methods. We try to evaluate BACTEC 9120 system capacity in order to detect of bacteremia due to Brucella spp and to confirm isolated bacteria by PCR. Blood culture sample of 102 suspected patients evaluated by BACTEC 9120 system. They were subcultured when the machine detected their growth; if not; blind subcultures were performed on days 7, 14, 21 and 28. Forty-one of 102 suspected patients showed bactermia. Isolation rate of Brucellawas 40.2%. All patients were detected by BACTEC 9120 system. All positive blood culture was detected via BACTEC 9120 and blind subcultures. No positive blood culture bottles were missed by the system. Our data obtained by using the BACTEC 9120 system indicates a more rapid detection of Brucella than conventional methods

Direct urease test and acridine orange staining on bactec blood culture for rapid presumptive diagnosis of brucellosis

Brucellosis is one of the most common zoonotic diseases in Iran and human brucellosis is endemic in all parts of the country. Growth of Brucella is slow and blood culture of these bacteria by use of classical methods is time-consuming. Furthermore, in endemic area culture is required for definitive diagnosis. In the present study, direct urease test and acridine orange staining were tried on the BACTEC blood culture broths for early presumptive identification of Brucella growth. Blood cultures were attempted in 102 seropositive patients. In the forty one blood cultures positive for Brucella, coccobacilli were seen in broth smears stained with acridine orange stain, and also were urease test positive, thus providing presumptive identification of Brucella growth. Urease test was negative and bacteria were not seen in the broth smears of the remaining 61 broths negative for Brucella growth. Because of simplicity, reliability and reproducibility, these tests can be routinely incorporated in the laboratory for diagnosis of brucellosis