RESUMEN
Glycoprotein from the eye lens of fish, Mystus cavasius, was isolated by extraction with 1% Triton X-100 in saline. The crude extract which was found to be electrophoretically heterogeneous was fractionated on a DEAE-cellulose column. One of the fractions obtained in major amount was further resolved by column chromatography using Sephadex G-150 into two homogeneous fractions (GP-1 and GP-2]. GP-1 contained carbohydrates (11.2%) and protein (77.5%). The constituent sugars were D-glucose, D-mannose, D-galactosamine and N-acetyl neuraminic acid. The principal amino acids were aspartic acid, serine, glutamic acid, glycine and alanine. The proportions of these residues were determined.
Asunto(s)
Aminoácidos/análisis , Animales , Carbohidratos/análisis , Cromatografía DEAE-Celulosa , Proteínas del Ojo/química , Peces/metabolismo , Glicoproteínas/química , Cristalino/química , Octoxinol , PolietilenglicolesRESUMEN
The excretory material (EM) was isolated from the culture medium of a virulent strain (F-2) of Pseudomonas solanacearum. Electrophoretic studies depicted the heterogeneous nature of EM and the presence of released lipopolysaccharide in it. Both the exopolysaccharide and the released LPS contained D-galactose as the major sugar constituent together with D-glucose, N-acetyl-D-galactosamine and N-acetyl-D-glucosamine. In addition, L-rhamnose was present as a constituent sugar of the released LPS.