RESUMEN
A study of the hematological parameters, glycogen, glucose, and lactate, and the activity of malate and lactate dehydrogenases was carried out in blood and tissues of fishes submitted to two, four, and six hours of hypoxia and recuperation. Only after 4 h of hypoxia was there a drop in liver glucose. After 6 h, a drop in lactate and a rise in glucose in practically all tissues signaled a recuperation of the metabolism, probably due to ASR (aerial surface respiration). Lactate formed during hypoxia was canalized to heart and brain for oxidation and used for neoglucogenesis. There were no changes in hematological parameters nore in the activity of malate and lactate dehydrogenases during normoxia and hypoxia, which suggest that these adaptive mechanisms may not be involved during hypoxia. Glycogen concentrations did not show variation during hypoxia either.
Foram estudados os parâmetros hematológicos, glicogênio, glicose e lactato, e a atividade da malato e do lactato desidrogenases em sangue e tecidos de peixes, em normóxia, com diferentes tempos de hipóxia (até seis horas) e recuperação. Apenas após quatro horas de hipóxia o fígado utiliza glicose. Em seis horas de hipóxia, a queda de lactato e o aumento de glicose em praticamente todos os tecidos indicam uma recuperação por meio da RAS (respiração aérea superficial), presumivelmente realizada. O lactato produzido em hipóxia é reaproveitado como combustível oxidativo no coração e no cérebro e como substrato para a neoglicogênese. Não houve variações entre normóxia e hipóxia nos parâmetros hematológicos e na atividade das enzimas LDH e MDH, o que sugere que, provavelmente, esses mecanismos adaptativos não estão envolvidos na resposta ao ambiente hipóxico. Não ocorre variação nas concentrações de glicogênio entre normóxia e hipóxia.
RESUMEN
A study of the hematological parameters, glycogen, glucose, and lactate, and the activity of malate and lactate dehydrogenases was carried out in blood and tissues of fishes submitted to two, four, and six hours of hypoxia and recuperation. Only after 4 h of hypoxia was there a drop in liver glucose. After 6 h, a drop in lactate and a rise in glucose in practically all tissues signaled a recuperation of the metabolism, probably due to ASR (aerial surface respiration). Lactate formed during hypoxia was canalized to heart and brain for oxidation and used for neoglucogenesis. There were no changes in hematological parameters nore in the activity of malate and lactate dehydrogenases during normoxia and hypoxia, which suggest that these adaptive mechanisms may not be involved during hypoxia. Glycogen concentrations did not show variation during hypoxia either