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Biocell ; 29(2): 187-193, ago. 2005. ilus
Artículo en Inglés | LILACS | ID: lil-429674

RESUMEN

Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAA was a mannose-binding lectin with three mannose-binding sites. Southern blot analysis of the genomic DNA revealed that zaa belonged to a multi-copy gene family


Asunto(s)
Lectina de Unión a Manosa/fisiología , Lectina de Unión a Manosa/genética , Lectina de Unión a Manosa/química , Lectina de Unión a Manosa , Proteínas de Plantas/fisiología , Proteínas de Plantas/genética , Proteínas de Plantas/química , Genes de Plantas/fisiología , Genes de Plantas/genética , Plantas Modificadas Genéticamente/fisiología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/química , Regulación de la Expresión Génica de las Plantas/fisiología , Regulación de la Expresión Génica de las Plantas/genética
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