Ocorrência de tuberculose em rebanhos bubalinos (Bubalus bubalis var. bubalis-Linneus, 1758) no Município de Parintins, Amazonas / Ocurrence of tubercullosis in herds of buffaloes of Amazonas State, Brazil

A comparative tuberculinization test was performed in 1303 animals from seven farms and 266 showed positive to the test. Mycobacterium bovis was isolated from a pool of lymphatic nodes from 14 reagents animals using Stonebrink media and confirmed by biochemical tests

Production and purification of epsilon prototoxin produced by Clostridium perfringens type D

A prototoxina epsilon foi obtida a partir de cultivo líquido, rico em carboidratos, a partir de amostra de C. perfringens tipo D (INTA - Argentina), incubado em condiçöes de anarobiose à 37ºC por oito horas. O sobrenadante da cultura foi concentrado utilizando-se o sistema Amicon com membrana de "cut off" de 10 kDa. O concentrado foi precipitado com sulfato de amônio (350 g/ml) e dissolvido em tampäo fosfato 0,01 M (pH 7,2). A prototoxina purificada por cromatografia de troca iônica (DEAE-Sepharose CL 6B) e isolada no primeiro pico, mostrou uma banda única de aproximadamente 34kDa de peso molecular em SDS-PAGE, além de ser tóxica em camundongos

Isolamento de Mycobacterium bovis em cäo / Mycobacterium bovis isolation in a dog

This report describes the isolation of Mycobacterium bovis from a dog with a history of co-habitation with bufallos infected with Mycobacterium bovis. After necropsy, the microrganism was isolated from a mesenteric lymphatic node in Stonebrink media and bacterial identification was confirmed by biochemical tests

Isolamento de Mycobacterium bovis em cão

This report describes the isolation of Mycobacterium bovis from a dog with a history of co-habitation with bufallos infected with Mycobacterium bovis. After necropsy, the microrganism was isolated from a mesenteric lymphatic node in Stonebrink media and bacterial identification was confirmed by biochemical tests.