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1.
Braz. j. med. biol. res ; 48(11): 1055-1062, Nov. 2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-762909

RESUMEN

Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction was performed by bone marrow implantation in both quadriceps of the animals, which were then divided into 3 groups: control (CG), transcutaneous electrical nerve stimulation (TENS) group (TG), and functional electrical stimulation (FES) group (FG) with 12 rats each. All animals were anesthetized and electrically stimulated twice per week, for 35 days from induction day. After this period, another blood sample was collected and quadriceps muscles were bilaterally removed for histological and calcium analysis and the rats were killed. Calcium levels in muscles showed significantly lower results when comparing TG and FG (P<0.001) and between TG and CG (P<0.001). Qualitative histological analyses confirmed 100% HO in FG and CG, while in TG the HO was detected in 54.5% of the animals. The effects of the muscle contractions caused by FES increased HO, while anti-inflammatory effects of TENS reduced HO.


Asunto(s)
Animales , Masculino , Osificación Heterotópica/terapia , Músculo Cuádriceps , Estimulación Eléctrica Transcutánea del Nervio , Antiinflamatorios , Compuestos Azo , Fosfatasa Alcalina/sangre , Trasplante de Médula Ósea , Estudios Transversales , Calcio/análisis , Modelos Animales de Enfermedad , Terapia por Estimulación Eléctrica , Eosina Amarillenta-(YS) , Verde de Metilo , Osificación Heterotópica/etiología , Osificación Heterotópica/patología , Músculo Cuádriceps/química , Músculo Cuádriceps/patología , Distribución Aleatoria , Ratas Wistar , Trasplante Autólogo
2.
Braz. j. med. biol. res ; 45(6): 497-501, June 2012. tab
Artículo en Inglés | LILACS | ID: lil-622784

RESUMEN

Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induced heterotopic bone formation have been proposed using heterologous demineralized bone matrix implants and bone morphogenetic protein and other tissues. The objective of the present experimental study was to identify a reliable protocol to induce HO in Wistar rats, based on autologous bone marrow (BM) implantation, comparing 3 different BM volumes and based on literature evidence of this HO induction model in larger laboratory animals. Twelve male Wistar albino rats weighing 350/390 g were used. The animals were anesthetized for blood sampling before HO induction in order to quantify serum alkaline phosphatase (ALP). HO was induced by BM implantation in both quadriceps muscles of these animals, experimental group (EG). Thirty-five days after the induction, another blood sample was collected for ALP determination. The results showed a weight gain in the EG and no significant difference in ALP levels when comparing the periods before and after induction. Qualitative histological analysis confirmed the occurrence of heterotopic ossification in all 12 EG rats. In conclusion, the HO induction model was effective when 0.35 mL autologous BM was applied to the quadriceps of Wistar rats.


Asunto(s)
Animales , Masculino , Ratas , Fosfatasa Alcalina/sangre , Trasplante de Médula Ósea/métodos , Osificación Heterotópica/etiología , Osteogénesis/fisiología , Músculo Cuádriceps , Biomarcadores/análisis , Trasplante de Médula Ósea/mortalidad , Calcio/análisis , Modelos Animales , Osificación Heterotópica/patología , Proyectos Piloto , Músculo Cuádriceps/química , Ratas Wistar , Espectrofotometría/métodos , Trasplante Autólogo , Aumento de Peso
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