Pharmacokinetic and tissue distributions study of adenosine, 4- hydroxybenzyl alcohol and Parishin C from Gastrodia elata extract in rats

The plant Gastrodia elata is a type of the orchid plant Gastrodia elata Bl. which contains glycosides, phenols, polysaccharides, sterols, and organic acids and a variety of active ingredients are proved to have certain pharmacological activities. To understand the process in the body of Gastridua elata, we used HPLC to study pharmacokinetics and tissue distributions of adenosine, 4-hydroxybenzyl alcohol and Parishin C in rats. The results showed that the three ingredients could be detected in plasma and different organizations at various time points. There was no significant difference in systemic clearance at three ingredients and it may be show that the three ingredients distributed [0.475+/-0.025, 0.518+/-0.033, 0.699+/-0.051] quickly and eliminated [5.37+/-0.87, 4.54+/-0.69, 5.34+/-0.82] slowly in plasma. There was the highest content of adenosine in spleen, followed by liver and lung. The highest content of 4-hydroxybenzylacohol in liver, and was higher in spleen. Parishin C was highest in heart, followed by liver and spleen. It is obvious that the contents of three ingredients are all higher in liver. The trends of the three ingredients' contents in G. rhizome extract were consistent with the contents in the plasma after intravenous administration

Application of quantitative evaluation on multicomponent drug interaction in anti-HIV research / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To quantitatively evaluate mutual relations of 4 component drugs in anti-HIV action.</p><p><b>METHODS</b>The effect of TCM four components on cell growth was detected using MTT assay. The antiviral effects of 4 components were observed at the maximal nonvenomous dose. The combination index (CI) value of combined two or four components were calculated using median-effect principle. The mutual relations of two or four components for antiviral actions were assessed using CI.</p><p><b>RESULTS</b>Synergism was dominant in combination of A and B, and the effect was dose-dependent. Antagonism was dominant in combination of C and D, and the effect was dose-dependent. But the combination of A, B, C, and D was synergistic when the inhibition rate was over 10%.</p><p><b>CONCLUSION</b>Median-effect principle can be used to quantitatively assess the anti-HIV effect of four components.</p>