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1.
Artículo en Chino | WPRIM | ID: wpr-1021300

RESUMEN

BACKGROUND:The elastic modulus of traditional bone implants is large and does not match the elastic modulus of human bone,which will cause a stress shielding effect and lead to bone resorption.The trabecular scaffold of the triply periodic minimal surface with radial gradient has elastic modulus matching with human cancellous bone,and its yield strength is greater than that of human cortical bone,which provides a new choice for the design of bone scaffold. OBJECTIVE:Triply periodic minimal surface structure with radial gradient was constructed by the implicit surface method.The sample was manufactured by laser selective melting technology,and the quasi-static compression test was carried out to obtain trabecular scaffolds with mechanical properties matching human bones. METHODS:Four types of the trabecular scaffolds of the triply periodic minimal surface with a radial gradient of G,I,P and D were established by the implicit surface method.Samples were manufactured by laser selective melting technology.We observed the surface morphology of the molded sample,evaluated the molding quality,conducted a quasi-static compression test,and evaluated the mechanical properties of the samples. RESULTS AND CONCLUSION:The quasi-static compression test results showed that compared with the four triply periodic minimal surface scaffolds,the platform stress of the G scaffold had less fluctuation and no failure or fracture,indicating that the G scaffold had the best plasticity.The mechanical properties of the G scaffolds with 45%,55%and 65%porosities were analyzed.It was found that the elastic modulus of G scaffolds with 55%porosity was within the range of elastic modulus of human cancellous bone(0.022-3.7 GPa),and the yield strength was close to the maximum yield strength of human cortical bone(187.7-222.3 MPa).In conclusion,G triply periodic minimal surface scaffold with 55%porosity can reduce the stress shielding effect,bear a higher body load,improve the stability of the implant,and prolong the service life of the implant.

2.
Artículo en Chino | WPRIM | ID: wpr-1022066

RESUMEN

BACKGROUND:Vertebral compression fracture is a common disease in the current orthopedic field.However,the occurrence of re-fracture in neighboring vertebrae after surgery is a problem that cannot be ignored,which has a serious impact on the normal life of patients. OBJECTIVE:The aim of this study is to establish four postoperative models with different recovery heights using computed tomography images.By using finite element analysis,we derived the stresses on the neighboring vertebrae at different recovery heights and further explored the importance of postoperative recovery of the height of the injured vertebrae. METHODS:A finite element model of the thoracolumbar spine(T11-L3)was established and validated,on the basis of which four postoperative finite element models of L1 with different recovery heights of 100%,80%,60%,and 40%were constructed,in which the cement capacity varied with the recovery height.The specific models are as follows:Model 1 was the postoperative model with normal recovery height,and the cement capacity was 8.3 mL.Model 2 was the postoperative model in which 20%of the anterior height of the L1 was removed and the posterior convexity angle became 10.41°,and the cement capacity was 6.9 mL.Model 3 was the postoperative model in which 40%of the anterior height of the L1 was removed and the posterior convexity angle became 20.17°,and the cement capacity was 4.7 mL.Model 4 was a postoperative model with 60%of the L1 anterior height removed and the posterior convexity angle changed to 28.85°,with a cement capacity of 3.6 mL.For evaluation of the postoperative model,we applied a moment of 7 Nm and an axial force of 500 N.The followings were recorded and analyzed:peak stresses in the L2 upper endplate and T12 lower endplate;peak stresses in the L2 and T12 cancellous bone. RESULTS AND CONCLUSION:(1)The highest peak stresses for each condition of the L2 upper endplate,T12 lower endplate,L2 cancellous bone,and T12 cancellous bone occurred in Model 1 and Model 4.In particular,the T12 lower endplate,except for the posterior extension condition,the anterior flexion,left and right lateral bending,and left and right rotation conditions all reached their highest peak stresses in Model 4,with stresses of 50.3,33.1,44.9,34.3,and 31.9 MPa.(2)Based on the peak stresses in the adjacent vertebral endplates and cancellous bone,after excluding Model 1 and Model 4,the minimum peak stresses for most of the conditions appeared in the Model 2,and the minimum peak stresses appeared in the Model 2 in 66.6%of the cases,especially in the upper endplates of the L2 and cancellous bone except for the posterior extension condition,the minimum peak stresses all appeared on the Model 2.(3)Therefore,controlling the recovery height at about 100%and 40%of the original height was a dangerous recovery height,which had a greater impact on the neighboring vertebrae.Controlling the recovery height at about 80%of the original height may be a more ideal choice.With a recovery height of about 80%of the original height,the adjacent vertebrae are subjected to less stress,thus reducing the risk of re-fracture of the adjacent vertebrae in the patient.

3.
Artículo en Chino | WPRIM | ID: wpr-928847

RESUMEN

This study introduces a non-invasive gastrointestinal early cancer magnetic anchor ablation electrode endoscopy system, including a magnetic anchor module and ablation electrode module. The magnetic anchoring module is composed of an external guide magnet and an internal magnet, made of permanent magnet material NdFeB; the ablation electrode module is based on the modification of the front end lens hood of the existing endoscope (CMD-90D LED electronic upper gastrointestinal endoscope). The new endoscope system not only includes all the functions of the original endoscope, but also introduces magnetic anchoring to enable the ablation electrode to be accurately positioned and controllable in the process of treating tumors, avoiding the phenomenon of gastric perforation; the introduction of steep pulse electric field ablation electrodes realizes the purpose of non-invasive treatment. Its clinical application will become a new method to treat early cancer of the digestive tract.


Asunto(s)
Humanos , Electrodos , Endoscopios , Tracto Gastrointestinal , Fenómenos Magnéticos , Neoplasias
4.
Artículo en Chino | WPRIM | ID: wpr-907421

RESUMEN

With the development of magnetic materials more widely used in surgical instruments, the development of magnetic surgery technology has continuously made breakthroughs and innovations. Magnetic anchor assist technology has become an important surgical method due to its precise positioning and non-contact traction. The majority of clinical magnetic anchor system consist a target magnet in vivo and a traction magnet in vitro. The non-contact traction and anchoring of the target can be achieved through the mechanical interaction of the anchor magnet and the traction magnet. This method not only can prevent the entanglement and collision between surgical instruments, but also can provide a good surgical field of vision for the surgical area, making the surgical process safer and more efficient. In this paper, 69 articles related to the application of magnetic anchor assist technology in surgical procedures were retrieved by conducting searches in the Chinese Journal Full-text Database(CNKI), Wanfang Database, VIP Chinese Sci-tech Journal Database, PubMed and other databases. After analysis and evaluation, a total of 36 papers were screened. The research progress and application status of magnetic anchor assist technology in combination with laparoscopy, thoracoscopy, and endoscopy in surgical operations were introduced. The results show that the magnetic anchor assist technology can greatly promote the development of minimally invasive surgery, but there are still problems in the application process, such as mutual interference of magnets and instability of the magnetic force, which still need to be further studied and improved.

5.
Artículo en Chino | WPRIM | ID: wpr-863229

RESUMEN

As a physical method, electromagnetic therapy has been widely used in clinical treatment of various human diseases. In this paper, a total of 123 research papers related to the clinical application of electromagnetic therapy were retrieved from the Chinese Journal Full-text Database also known as China National Knowledge Infrastructure (CNKI), WANFANG Database, Chinese Science and Technology Journal Database (CSTJ), PubMed, Europe PMC, etc. All papers were analyzed and evaluated, and 58 effective articles were selected. The clinical application and therapeutic effects of electromagnetic therapy on several diseases were reviewed, including hypertension, diabetes, insomnia, depression and osteoporosis. The categories of commonly used electromagnetic therapy were sorted out and summarized, including magnetization at acupoints, pulsed electromagnetic fields, gyromagnetic, repetitive transcranial magnetic stimulation, and wearing magnetic items. The results show that electromagnetic therapy has a wide range of applications and small side effects, it has significant effects on some medical diseases, neurological diseases, and orthopedic diseases.

6.
Artículo en Inglés | WPRIM | ID: wpr-776903

RESUMEN

Danshen, the dried root or rhizome of Salvia miltiorrhiza Bge., is a traditional and folk medicine in Asian countries, especially in China and Japan. In this review, we summarized the recent researches of Danshen in traditional uses and preparations, chemical constituents, pharmacological activities and side effects. A total of 201 compounds from Danshen have been reported, including lipophilic diterpenoids, water-soluble phenolic acids, and other constituents, which have showed various pharmacological activities, such as anti-inflammation, anti-oxidation, anti-tumor, anti-atherogenesis, and anti-diabetes. This article intends to provide novel insight information for further development of Danshen, which could be of great value to its improvement of utilization.


Asunto(s)
Diterpenos , Química , Medicamentos Herbarios Chinos , Química , Farmacología , Usos Terapéuticos , Hidroxibenzoatos , Química , Estructura Molecular , Aceites Volátiles , Química , Fitoquímicos , Química , Farmacología , Usos Terapéuticos , Raíces de Plantas , Química , Control de Calidad , Salvia miltiorrhiza , Química
7.
Artículo en Chino | WPRIM | ID: wpr-1008250

RESUMEN

The project was launched to analyze the effects of sulfur-fumigated Ophiopogonis Radix on endogenous metabolites in rats by metabonomics. The preparation method of sulfur-fumigated Ophiopogonis Radix in laboratory was established. Then the blood samples of SD rats in blank group,Ophiopogonis Radix extract group and sulfur-fumigated Ophiopogonis Radix extract group were investigated by UHPLC-Q-Exactive. The differential metabolites were screened and identified by PCA(principal component analysis),OPLSDA(orthogonal partial least squares discriminant analysis) and variable importance projection(VIP),and the metabolic pathways were analyzed. Finally,a total of 15 potential biomarkers were identified. Compared with the samples of Ophiopogonis Radix extract group,sulfur-fumigated Ophiopogonis Radix mainly affected the biosynthesis and metabolism of amino acids in normal rats. Its mechanism may be related to the biosynthesis of phenylalanine,tyrosine,tryptophan and aminoacyl-tRNA as well as the metabolism of phenylalanine and tryptophan. Based on UHPLC-HRMS metabonomics,this paper discussed the effects of sulfur-fumigated Ophiopogonis Radix on endogenous metabolites in rats,which provided an idea for the metabolic study of other sulfur-fumigated traditional Chinese medicines.


Asunto(s)
Animales , Ratas , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos , Metabolómica , Ratas Sprague-Dawley , Azufre
8.
Artículo en Chino | WPRIM | ID: wpr-775394

RESUMEN

To identify the metabolites of Danshensu in plasma and urine in rats by using UHPLC-LTQ-Orbitrap method. After oral gavage of Danshensu CMC-Na suspension in SD rats, urine and plasma samples were collected and processed by solid phase extraction. ACQUITY UPLC BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm) was utilized, with 0.1% formic acid (A)-acetonitrile (B) solution as the mobile phase for gradient elution. Negative electrospray ion mode based data-acquisition method was established to collect the mass spectrometry data of biological samples. As a result, Danshensu and 21 Danshensu Ⅰ phase and Ⅱ phase metabolites were finally identified according to the accurate mass measurements, mass fragmentation behaviors and comparing with the reference standards. The main metabolic pathways included dehydration, methylation, glucuronide conjugation, sulfate conjugation and their composite reactions. Consequently, our study expounded metabolites of Danshensu in rats based on UHPLC-LTQ-Orbitrap method and provided a reference for further researches on therapeutic material basis and mechanism of Danshensu.


Asunto(s)
Animales , Ratas , Cromatografía Líquida de Alta Presión , Lactatos , Sangre , Metabolismo , Orina , Espectrometría de Masas , Ratas Sprague-Dawley
9.
Artículo en Chino | WPRIM | ID: wpr-776406

RESUMEN

An efficient method of ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap (UHPLC-LTQ-Orbitrap) mass spectrometer was established to elucidate the metabolites of tanshinone Ⅰ and tanshinone ⅡA in rats. Urine and plasma samples were collected after oral gavage. After processing biological sample by solid phase extraction, Waters ACQUITY HPLC BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm) was used with 0.1% formic acid (A) - acetonitrile (B) solution as the mobile phase for gradient elution. The plasma, urine and the blank samples were then analyzed by ESI-LTQ-Orbitrap equipped with an ESI ion source under positive ion mode. On the basis of the accurate mass measurements, multiple mass spectra and comparison of data with published literature, a total of 26 metabolites were tentatively identified and characterized in the rat samples. Among them, 7 metabolites were derived from tanshinone Ⅰ through metabolic pathways of glucuronide conjugation, hydroxylation, reduction reaction, demethylation reaction, methylation, sulfate conjugation and their composite reactions. Nineteen metabolites were derived from tanshinone ⅡA through metabolic pathways of hydroxylation, reduction reaction, methylation, sulfate conjugation, glucuronidation, glucosylation and their complicated reactions. The results showed that the metabolism of tanshinone Ⅰ and tanshinone ⅡA in rats could be comprehensively clarified by using UHPLC-LTQ-Orbitrap mass spectrometer, providing material basis for the further research in terms of pharmacodynamics, toxicology, and secondary development of Chinese medicine.


Asunto(s)
Animales , Ratas , Cromatografía Líquida de Alta Presión , Abietanos , Sangre , Metabolismo , Orina
10.
Artículo en Inglés | WPRIM | ID: wpr-773630

RESUMEN

Sesquiterpene lactones are considered as the major active compounds in Kudiezi injection in virtue of their special structures and activities. Herein, an analytical method was developed for rapid screening and identification of sesquiterpene lactones in Kudiezi injection using high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry (HPLC-LTQ-Orbitrap) in negative ion mode. First, two sesquiterpene lactone reference standards were analyzed to obtain their characteristic ESI-MS/MS fragmentation patterns. Second, based on extracted ion chromatography (EIC) data-mining method and characteristic fragmentation pathways analysis, sesquiterpene lactones in Kudiezi injection were rapidly screened and identified. Finally, an important parameter Clog P was adopted to discriminate the isomers of sesquiterpene lactones. As a result, 50 sesquiterpene lactones were characterized, including 9 sesquiterpene lactone aglycones, 39 sesquiterpene lactone glycosides, and 2 amino acid-sesquiterpene lactone conjugates. Among them, 13 compounds were tentatively identified as new compounds. The results demonstrated that the established method would be a rapid, effective analytical tool for screening and identification of sesquiterpene lactones in the complex system of natural medicines.


Asunto(s)
Cromatografía Líquida de Alta Presión , Métodos , Medicamentos Herbarios Chinos , Química , Isomerismo , Lactonas , Química , Sesquiterpenos , Química , Espectrometría de Masas en Tándem , Métodos
11.
Artículo en Chino | WPRIM | ID: wpr-664889

RESUMEN

The melamine molecular imprinted polymer ( MIP ) membrane immobiliZed on the surface of the surface plasmon resonance ( SPR) chip was prepared with allylcyanurate and ethylene glycol dimethacrylate as a functional monomer and cross-linker, respectively.Based on the ratio of the intercept to the slope of Langmuir equation for imprinted polymer, the association constant ( Kass ) of imprinted sites in the imprinted film to melamine was calculated to be 1.40 × 108 L/mol.The SPR sensor showed high selectivity to the template molecule melamine, and the best response was obtained at pH=7.5.The method showed good linearity in the melamine concentration range of 0.1-10.0 nmol/L ( R=0.9991 ).Based on a signal to noise ratio of 3, the detection limits of melamine were 62.6 pmol/L and 56.4 pmol/L for the milk and milk powder, respectively.The recoveries were 91.5% for milk and 92.0% for milk powder.These results suggest that SPR sensing combined with MIP film is a promising alternative method for detection of melamine.

12.
Artículo en Chino | WPRIM | ID: wpr-689792

RESUMEN

Cervical spondylosis is due to degenerative cervical disc and its stimulation or oppression of the adjacent nerves, spinal cord, spinal artery and other tissue caused by clinical symptoms. The cervical spine is an anatomical structure with activity, while the pillow has a certain plastic fixation effect on the cervical spine anatomy. Therefore, the pillow not only plays a health role in the cervical spine, but also plays an important role in restoring the normal physiological curvature of the cervical spine. Based on this, a multi-functional cervical vertebra treatment pillow is designed, which not only has the functions of traction, maintaining different positions of the cervical spine, correcting the cervical curvature and equipment exercises, but also has the functions of voice broadcast and network data terminal.

13.
Artículo en Chino | WPRIM | ID: wpr-689796

RESUMEN

In the research of artificial joint biomechanics, it is a common method in the world to evaluate the biomechanical properties of the implanted fixtures through experiments . The domestic research started late, and the corresponding testing software were lacking. There is still no special software. In this paper, an integrated software test system was designed and built based on the existing hardware system, including:hardware control, data collection, data save, data processing and display. It can directly show the 3D motion trajectory and the angle curve of joints. The system can accurately measure the relative motion angle value, external torque value, and inter pressure value of each joint. It has some reference significance for the development of the artificial joints' evaluation system.

14.
Artículo en Chino | WPRIM | ID: wpr-689817

RESUMEN

In the research of artificial joint biomechanics, it is a common method in the world to evaluate the biomechanical properties of the implanted fixtures through experiments . The domestic research started late, and the corresponding testing methods were lacking. There is still no unified standard. In this paper, a complete hardware test system was designed and built around the existing mechanical testing machine, including:binocular vision catcher, torque bearing clamp, film pressure sensor and so on. The system can accurately measure the relative motion angle value, external torque value, and inter pressure value of each joint. It has some reference significance for the forming and standardization of the artificial joints' evaluation system.


Asunto(s)
Fenómenos Biomecánicos , Prótesis Articulares , Movimiento (Física) , Presión , Torque
15.
Artículo en Inglés | WPRIM | ID: wpr-812420

RESUMEN

Sesquiterpene lactones are considered as the major active compounds in Kudiezi injection in virtue of their special structures and activities. Herein, an analytical method was developed for rapid screening and identification of sesquiterpene lactones in Kudiezi injection using high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry (HPLC-LTQ-Orbitrap) in negative ion mode. First, two sesquiterpene lactone reference standards were analyzed to obtain their characteristic ESI-MS/MS fragmentation patterns. Second, based on extracted ion chromatography (EIC) data-mining method and characteristic fragmentation pathways analysis, sesquiterpene lactones in Kudiezi injection were rapidly screened and identified. Finally, an important parameter Clog P was adopted to discriminate the isomers of sesquiterpene lactones. As a result, 50 sesquiterpene lactones were characterized, including 9 sesquiterpene lactone aglycones, 39 sesquiterpene lactone glycosides, and 2 amino acid-sesquiterpene lactone conjugates. Among them, 13 compounds were tentatively identified as new compounds. The results demonstrated that the established method would be a rapid, effective analytical tool for screening and identification of sesquiterpene lactones in the complex system of natural medicines.


Asunto(s)
Cromatografía Líquida de Alta Presión , Métodos , Medicamentos Herbarios Chinos , Química , Isomerismo , Lactonas , Química , Sesquiterpenos , Química , Espectrometría de Masas en Tándem , Métodos
16.
Artículo en Chino | WPRIM | ID: wpr-618435

RESUMEN

Chemotherapy is one of the traditional tumors treatment solutions.Chemotherapy has the feature of tissue non-specificity,which can cause side effects on normal cells while inhibiting tumor cell growth.Magnetic targeting drug delivery system (MTDDS) employs biocompatible and stable magnetic nanoparticles (MNP) as drug carries to transport and accumulate anticancer drugs to the specific tumor tissues under the guidance of external magnetic field.This technology not only improves the efficiency of drug delivery and antitumor activity,but also reduces the drug dosage and side effects.The properties of drug-loaded MNPs and the applied external magnetic field are the main factors that affecting the MNPs targeting to the tumor tissues.The effectiveness of the targeted delivery of the drug-loaded magnetic nanoparticles mainly depends on the form and strength of the magnetic field at the target site.That is,whether there is sufficient strength to attract and retain NMPs,and to promote antitumor drug release at the tumor region.In this paper,the research progress of static magnetic field targeting drug delivery system in tumor diagnosis and therapy was summarized,which can provide some basic information for the relative scientific researches.

17.
Chinese Journal of Neonatology ; (6): 213-216, 2017.
Artículo en Chino | WPRIM | ID: wpr-610423

RESUMEN

Objective To study the differences of coagulation indices on the first day of birth in newborns with different gestational ages.Method Premature infants born in our hospital between January 2014 and December 2015 were enrolled in this study as the observational group,and they were divided into early preterm group,moderate preterm group,and late preterm group according to their gestational ages.Healthy full-term infants born during the same period were selected as the control group by 3:1 The clinicaldata and coagulation indices of the infants and their mothers in each group were compared.Result There were 44,50,71,and 52 cases in the early preterm,moderate preterm,late preterm,and control group,respectively.The prothrombin time (PT),activated partial thromboplastin time (APTT),and thrombin time (TT) of the premature infants in the early preterm group,moderate preterm group,and late preterm group were all longer than those of the control group [PT:(16.1 ±4.3) s,(16.8 ±4.9) s,(15.8 ±4.8) s,vs.(13.0±1.3)s;APTT:(88.3±38.1) s,(93.5±37.7) s,(91.0±32.3) s,vs.(66.0±17.8) s;TT:(25.4 ±4.6) s,(25.1 ±5.5) s,(25.0 ±3.3) s,vs.(24.0 ±3.3) s;all P<0.05].The fibrinogen level of the premature newborns in three groups were all lower than that of the full-term infants in the control group [(1.11 ± 0.46) g/L,(1.12 ± 0.44) g/L,(1.12 ± 0.45) g/L vs.(1.28 ± 0.37) g/L,P < 0.05].The differences of all the indices among the three groups of premature infants were all not statistically significant (P > 0.05).The comparison of the coagulation indices of the mothers of the newborns from all four groups showed no significant differences (P > 0.05).Conclusion Compared with full-term infants,preterm infants showed significantly poorer coagulation function on the first day of birth.However,there were no significant differences in coagulation indices among preterm infants of different gestational ages.

18.
Journal of Preventive Medicine ; (12): 226-230,235, 2017.
Artículo en Chino | WPRIM | ID: wpr-792597

RESUMEN

Objective To explore the association between different visual display terminal use and physical symptoms among adults.Methods Hangzhou,Jiaxing,Huzhou,Jinhua and Quzhou City were selected,where residents aged 18 years old and above were investigated using Mitofsky-Waksberg two-stage sampling.Information of demography,different visual display terminal use,eye and musculoskeletal symptoms,eye disease and eye relax were collected.Backward stepwise logistic regression was used to explore the association between different visual display terminal use and the symptoms (P value for addition < 0.05,P value for removal > 0.1).Results Totally 350 adults responded to the investigation,with a response rate of 48.54%.Finally,333 respondents were included in the analysis,among which 120 reported eye symptoms (36.04%) and 71reported musculoskeletal symptoms (21.32%).More time of computer use at work(0.5-4 h:OR=2.006,95% CI:1.021-3.943;4-6 h:OR=3.578,95% CI:1.751-7.315;>6 h:OR=4.874,95% CI:1.897-12.521) and eye disease (OR =4.361,95% CI:2.465-7.714) were significandy associated with higher odds ratios in eye symptoms.Similar pattern was seen for the association on time of computer use at work with musculoskeletal symptoms (0.5-4 h:OR =3.815,95 % CI:1.809-8.045;4-6 h:OR =4.974,95 % CI:2.281-10.845;> 6 h:OR =5.934,95% CI:2.347-15.005).More time of watching TV on television (> 2 h:OR =2.051,95%CI:1.068-3.939) and women (OR =1.884,95% CI:1.052-3.372) were also observed to be statistically associated with musculoskeletal symptoms.However,no statistical association was observed between the two symptoms and entertainment purpose use of computer/cell phone/pad/kindle (P > 0.05).Conclusion Computer use at work was significantly associated with eye symptoms,especially for those having eye diseases.It was also statistically associated with musculoskeletal symptoms,especially for women.

19.
Artículo en Inglés | WPRIM | ID: wpr-812035

RESUMEN

Kudiezi injection has been used extensively in the treatment of cerebrovascular and cardiovascular diseases. However, its therapeutic effects and underlying mechanism of action are not fully understood. The aim of the present study was to clarify the protective mechanisms of Kudiezi injection on cerebral ischemic injury, using metabolomics methods. Middle cerebral artery occlusion (MCAO) was introduced in rats to build the cerebral ischemic damage. UHPLC-LTQ-Orbitrap-based analytical method was established for analysis of the metabolites. The raw mass data of all samples were normalized with Sieve 2.2 software and then introduced to orthogonal partial least squares discriminant analysis (OPLS-DA) model. Finally, 23 metabolites in plasma (15 were tentatively identified) were chosen as potential biomarkers, according to accurate mass measurements (< 5 ppm), MS/MS fragmentation patterns, and diagnostic product ions. Furthermore, on the basis of metabolic pathway analysis via metabolomics pathway analysis (MetPA), we first discovered that the protection mechanism in anti-ischemic cerebral reperfusion damage of Kudiezi injection was possibly related to the biosynthesis of phenylalanine, tyrosine, and tryptophan. The present study provided a useful approach for exploring the mechanism of ischemic stroke and evaluating the efficacy of Kudiezi injection or other traditional medicines.


Asunto(s)
Animales , Masculino , Ratas , Asteraceae , Química , Biomarcadores , Sangre , Isquemia Encefálica , Sangre , Quimioterapia , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos , Farmacología , Usos Terapéuticos , Inyecciones , Redes y Vías Metabólicas , Metabolómica , Extractos Vegetales , Farmacología , Usos Terapéuticos , Ratas Sprague-Dawley , Daño por Reperfusión , Sangre , Quimioterapia
20.
Artículo en Inglés | WPRIM | ID: wpr-812054

RESUMEN

Liriope (Liliaceae) species have been used as folk medicines in Asian countries since ancient times. From Liriope plants (8 species), a total of 132 compounds (except polysaccharides) have been isolated and identified, including steroidal saponins, flavonoids, phenols, and eudesmane sesquiterpenoids. The crude extracts or monomeric compounds from this genus have been shown to exhibit anti-tumor, anti-diabetic, anti-inflammatory, and neuroprotective activities. The present review summarizes the results on phytochemical and biological studies on Liriope plants. The chemotaxonomy of this genus is also discussed.


Asunto(s)
Animales , Humanos , Antiinflamatorios , Farmacología , Antineoplásicos Fitogénicos , Farmacología , Flavonoides , Farmacología , Hipoglucemiantes , Farmacología , Liriope (Planta) , Química , Medicina Tradicional , Fármacos Neuroprotectores , Farmacología , Fenoles , Farmacología , Fitoterapia , Extractos Vegetales , Farmacología , Saponinas , Farmacología , Sesquiterpenos , Farmacología
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