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Objective:To establish the HPLC fingerprint of Centellae herba and determine the content of asiaticoside, madecassic acid and asiaticoside B simultaneously; To compare the quality differences of Centellae herba collected in different months. Methods:The chromatographic condition was a Shimadzu InertSustain C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile and 2 mmol/L beta cyclodextrin in gradient elution at the flow rate of 0.8 ml/min. The detection wavelength was 204 nm, and the column temperature was 30 ℃. The different Centellae herba materials of collected in 2-12 months from Chenzhou were studied by the similarity evaluation combined with cluster analysis, principal component analysis and the three contents determination. Results:The HPLC fingerprint of Centellae herba was established and 9 common peaks were designated. The eleven samples were different, which can be aggregated into 4 categories and the quality of Centellae herba collected in July was the best. Conclusion:The established fingerprint and multi-components quantitative method are stable and reliable, which can provide a reference for the quality control and the utilization of Centellae herba resource.
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ObjectiveTo investigate the difference in the distribution frequency of APOBEC3B copy number variations (CNVs) between patients with different outcomes after hepatitis B virus (HBV) infection and the role of APOBEC3B CNVs in clinical outcome. MethodsA total of 296 patients with acute self-limiting recovery after HBV infection and 819 patients with different stages of chronic HBV infection who visited The First Affiliated Hospital of Anhui Medical University from January 2016 to December 2017 were enrolled as acute self limiting recovery group and chronic HBV infection group, respectively, and their peripheral blood samples were collected. Among the 819 patients with chronic HBV injection, 444 had chronic hepatitis B (CHB), 252 had liver cirrhosis (LC), and 123 had hepatocellular carcinoma (HCC). The AccuCopy method was used to measure APOBEC3B CNVs in peripheral blood, and related clinical data were collected for all patients. The chi-square test was used for comparison of distribution frequency of APOBEC3B CNVs between groups. ResultsFor acute or chronic outcome after chronic HBV infection, the acute self limiting recovery group had a significantly lower proportion of patients with reduced or deleted APOBEC3B CNVs than the chronic HBV infection group (46.96% vs 58.00%, P=0.001 1). For disease progression after chronic HBV infection, the HCC group had the highest proportion of patients with deleted APOBEC3B CNVs of 22.76%, followed by the LC group (14.29%) and the CHB group (11.04%), and there was a significant difference between the three groups (χ2=11.85, P=0019). In the chronic HBV infection group, there was no significant difference in the distribution frequency of APOBEC3B CNVs between the patients with positive E-antigen and those with negative E-antigen(χ2=0639,P=0727). ConclusionAPOBEC3B CNV is associated with the outcome of chronic HBV infection, and reduced and deleted APOBEC3B CNV is a genetic susceptibility factor for chronicity and progression of HBV infection.
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ObjectiveTo investigate the difference in the distribution frequency of APOBEC3B copy number variations (CNVs) between patients with different outcomes after hepatitis B virus (HBV) infection and the role of APOBEC3B CNVs in clinical outcome. MethodsA total of 296 patients with acute self-limiting recovery after HBV infection and 819 patients with different stages of chronic HBV infection who visited The First Affiliated Hospital of Anhui Medical University from January 2016 to December 2017 were enrolled as acute self limiting recovery group and chronic HBV infection group, respectively, and their peripheral blood samples were collected. Among the 819 patients with chronic HBV injection, 444 had chronic hepatitis B (CHB), 252 had liver cirrhosis (LC), and 123 had hepatocellular carcinoma (HCC). The AccuCopy method was used to measure APOBEC3B CNVs in peripheral blood, and related clinical data were collected for all patients. The chi-square test was used for comparison of distribution frequency of APOBEC3B CNVs between groups. ResultsFor acute or chronic outcome after chronic HBV infection, the acute self limiting recovery group had a significantly lower proportion of patients with reduced or deleted APOBEC3B CNVs than the chronic HBV infection group (46.96% vs 58.00%, P=0.001 1). For disease progression after chronic HBV infection, the HCC group had the highest proportion of patients with deleted APOBEC3B CNVs of 22.76%, followed by the LC group (14.29%) and the CHB group (11.04%), and there was a significant difference between the three groups (χ2=11.85, P=0019). In the chronic HBV infection group, there was no significant difference in the distribution frequency of APOBEC3B CNVs between the patients with positive E-antigen and those with negative E-antigen(χ2=0639,P=0727). ConclusionAPOBEC3B CNV is associated with the outcome of chronic HBV infection, and reduced and deleted APOBEC3B CNV is a genetic susceptibility factor for chronicity and progression of HBV infection.