Studies on transplanting suspension-cultured protocorms of Dendrobium candidum onto solid culture medium / 中国中药杂志

<p><b>OBJECTIVE</b>The suspension-cultured protocorms of Dendrobium candidum were transplanted on the solid culture medium for studying the factors influencing their differentiation and growth.</p><p><b>METHOD</b>The growth and differentiation of protocorms were detected when different base-media, plant phytohormones, carbohydrates and pH were applied.</p><p><b>RESULT AND CONCLUSION</b>The diluted MS medium promotes the growth of protocorms but inhibit differentiation. The phytohormone NAA, IAA, IBA and GA are helpful to the growth and differentiation of protocorms, however, the low pH values and presence of autoclaved fungi have suppressive effects on protocorms. The fungi also induce morphological variation of protocorms.</p>

INFLUENCES OF FUNGUS MYCENAE SP.ON ACTIVITIES OF SOME ENZYMES AND EXTRACELLULAR PH OF PROTOCORMS OF DENDROBIUM CANDIDUM / 微生物学通报

An elicitor from fungus Mycenae sp. enhanced the extracellular pH of protocorms of Dendrobium candidum in two stages and also inspired the activities of PAL, POD and LOX. The different elicitors were different in enhancing the pH. The activities of PAL and POD ascended twice after elicitors were applied. The protocorms treated twice by elicitor had the higher PAL activity.