RESUMEN
1,3-propanediol production by microbial fermentation has become the research hot spot for its amiability with the environment. Here the molecular mechanism of glycerol bioconversion to 1,3-propanediol was outlined by elucidating the fermentation strains, metabolic pathways, regulon and key enzymes. Of enzymes, glycerol dehydrogenase, the velocity-limiting enzyme in glycerol reductive pathway, was emphatically discussed with regard to its molecular structure and reactivating factors. This paper aims to provide the basis for genetic modification of fermentation strains.
Asunto(s)
Bacterias , Genética , Metabolismo , Proteínas Bacterianas , Genética , Metabolismo , Vías Biosintéticas , Fermentación , Orden Génico , Glicerol , Metabolismo , Hidroliasas , Genética , Metabolismo , Microbiología Industrial , Métodos , Glicoles de Propileno , Metabolismo , Deshidrogenasas del Alcohol de Azúcar , Genética , MetabolismoRESUMEN
Hyaluronic acid(HA)is a linear polysaccharide chain composed of alternating ?-1,4-glucuronic acid(GlcA)and ?-1,3-N-acetylglucosamine(GlcNAc)moieties.Construction of engineering strain has become the prevailing strategy for increasing yield and improving its quality,especially the molecular weight.Here the molecular mechanism of HA biosynthesis in Streptococcus strains was reviewed,involving fermentation strains,operon structure,crucial enzyme and construction of engineering strains.In addition,the prevalent problems in HA fermentation production were also discussed and the protocols were tentatively put forward for the upcoming research and industrial production.