Anticancer Effect and Molecular Docking Mechanism of 24-OH-panaxadiol / 中国实验方剂学杂志

Objective:To investigate the effect of ginsenoside 20(S),25-epoxydammarane-3β,12β,24α-triol (24-OH-panaxadiol,24-OH-PD) on inhibiting proliferation and inducing apoptosis of tumor cells, and explore its mechanism of action. Method:The inhibitory effect of 24-OH-PD (12.5,25,50,100 mg·L-1) on proliferation of CCRF-CEM, M14, MD-MBA-231 and Jeko-1 cells with different treatment periods (24, 48,72 h) was evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT)assay and CellTiter Glo test, and the results were then compared with 20(R)-Rg3 and 20(S)-Rh2.Flow cytometry was used to detect cell apoptosis caused by 24-OH-PD. Besides, the potential anticancer mechanism was studied by docking analysis with 40 cancer related proteins and 24-OH-PD by using drug design platform Schrodinger Maestro 6.7 Software. Result:24-OH-PD inhibited the proliferation of all the 4 cancer cell lines significantly in a time and dosage dependent manner. The IC50 value of 24-OH-PD on CCRF-CEM,Jeko-1,M14,and MD-MBA-231 cell lines was 25.36, 39.29, 21.74, and 19.35 mg·L-1, respectively, similar to 20(S)-Rh2 (IC50 23.35, 65.79, 18.95, 19.67 mg·L-1) and much better than 20(R)-Rg3 (only effective for Jeko-1 cells, IC50 49.5 mg·L-1). Annexin V/PI double staining experiment showed that 24-OH-PD could also induce apoptosis of the 4 kinds of cancer cells (PConclusion:24-OH-PD could inhibit cell proliferation and induce apoptosis for CCRF-CEM, M14, MD-MBA-231 and Jeko-1 cell lines, which may through directly acting on Bcl-2, Bcl-xl, and other proteins.

Comparison of Volatile Oil of Domestic and Foreign Panacix Quinquefolii Radix by HS-SPME/GC-MS / 中国实验方剂学杂志

Objective: To determine the volatile chemical constituents of Panacix Quinquefolii Radix of different origins and different growth years,in order to provide the theoretical basis for the further development and utilization of Panacix Quinquefolii Radix. Method: Headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to extract,analyze and identify the volatile constituents of Panacix Quinquefolii Radix. The chromatographic peak area normalization method was used to determine the relative amount of ingredients. Result: A total of 151 compounds were identified in Panacix Quinquefolii Radix samples,including 99 hydrocarbons,21 alcohol phenols,7 aldehydes,8 ketones,1 ester,15 heterocyclics and other compounds. Totally 68 kinds of compounds were identified in the roots of three-year-old Jilin Baishan,and the mass fraction accounted for 98.27%,67 compounds were identified in the roots of four-year-old Jilin Baishan,and the mass fraction accounted for 98.79%,65 compounds were identified in 3-year-old Panacix Quinquefolii Radix root,and the mass fraction accounted for 95.81%,and 63 compounds were identified in 4-year-old Panacix Quinquefolii Radix root,and the mass fraction accounted for 99.67%. The specific components of the volatile oil components of the four Panacix Quinquefolii Radix samples were 24,23,19,and 23,respectively,and the total composition was 16 species. Conclusion: The content and composition of volatile chemical constituents of Panacix Quinquefolii Radix of different origins are very different. This experiment provides a reference for the future quality evaluation of Panacix Quinquefolii Radix medicinal materials,rational development and utilization of resources.

Absorbed components analysis of Zhitong Huazheng Capsules in rat serum by UPLC-Q-TOF/MS / 中国中药杂志

To identify and analyze the constituents in rat serum after oral administration of Zhitong Huazheng capsule (ZTHZC), and provide a reference for its further research on pharmacodynamics material basis. Female Wistar rats were selected as experimental animals, and received intragastric administration of ZTHZC at a dose of 1.5 g·kg⁻¹. After the serum samples were collected, the absorbed prototype components in rat serum were identified and analyzed by using ultra-high performance liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) combined with multivariate statistical analysis．The results showed, a total of fifteen absorbed constituents were identified, all of which were prototype components, including Danshensu, salvianolic acid A, B, C, D, 9,12-dihydroxy-15-nonadecanoicacid, linoleic acid, ethyl palmitoleate, tetrahydropalmatine, fumarate A, astragaloside A, astragaloside II, saponin, locustin and luteolin. This experiment showed that these fifteen components absorbed into blood may be the potential bioactive components in ZTHZC, providing a scientific basis for clarifying its material basis in pharmacodynamics.

Application value of procalcitonin, cholinesterase and cardiac troponin Ⅰ in the evaluation of disease degree and prognosis of sepsis patients / 新乡医学院学报

Objective To investigate the application value of procalcitonin (PCT),cholinesterase (CHE) and cardiac troponin Ⅰ (cTnⅠ) in the evaluation of disease degree and prognosis of sepsis patients,so as to provide the basis for clinical diagnosis and prognostic evaluation of sepsis.Methods A total of 114 patients with sepsis were selected in Huanggang Central Hospital from May 2014 to June 2016.The patients were divided into sepsis group (n =47),severe sepsis group (n =42) and septic shock group (n =25) according to the severity of the disease.The patients were divided into death group (n =23) and survival group (n =91) according to the survival status.The levels of serum PCT,CHE and cTnⅠ were compared in the groups.Results The levels of serum PCT and cTnI in septic shock group were significantly higher than those in severe sepsis group and sepsis group (P ＜ 0.05).The levels of serum PCT and cTnⅠ in severe sepsis group were significantly higher than those in sepsis group (P ＜ 0.05).The level of serum CHE in septic shock group was significantly lower than that in severe sepsis group and sepsis group (P ＜ 0.05),and the serum CHE level in severe sepsis group was significantly lower than that in sepsis group (P ＜ 0.05).The levels of serum PCT and cTnI in the death group were significantly higher than those in the survival group (P ＜ 0.05),and the serum CHE level in the death group was significantly lower than that in the survival group (P ＜ 0.05).Conclusion The levels of serum PCT,CHE and cTnⅠ are important in evaluating the severity and prognosis of patients with sepsis.

Study on excretion of pseudo-ginsenoside GQ / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the pseudo-ginsenoside GQ (PGQ) in rat bile, feces and urine, and to study on the excretion of pseudo-ginsenoside GQ.</p><p><b>METHOD</b>Reverse phase high-performance liquid chromatography (RP-HPLC) method with an evaporative light-scattering detector (ELSD) was performed on Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol-water (24: 7) with flow rate of 1.0 mL x min(-1). ELSD parameters were set as follows: nitrogen gas pressure 3.0 bar, drift tube temperature 50 degrees C.</p><p><b>RESULT</b>The method fulfilled all the standard requirements of precision, accuracy and linearity. The main way of excretion of PGQ in rat administrated through sublingual vein was at the bile. The bile excretion ratio of PGQ was 41.60%, and feces excretion ratio was 9.97%. Only trace amount of PGQ was excreted in urine.</p><p><b>CONCLUSION</b>Almost all unchanged PGQ was excreted in bile, feces and urine.</p>

Distribution and pharmacokinetics of 20S-ginsenoside Rg3 eyeointment in rabbit eye / 药学学报

<p><b>AIM</b>To determine 20S-ginsenoside Rg3 in various tissues of rabbit eye and evaluate pharmacokinetics following its topical application.</p><p><b>METHODS</b>45 rabbits were divided into 9 groups. Aqueous humor, cornea and iris-ciliary body were collected at 0.017, 0.033, 0.083, 0.167, 0.5, 1.0, 1.5, 2.0 and 3.0 h following topically applying 1% 20S-ginsenoside Rg3 eyeointment and the concentrations in eye tissues were measured by HPLC.</p><p><b>RESULTS</b>The pharmacokinetics of 20S-ginsenoside Rg3 in rabbit eye were described by one-compartment model. The peak times in aqueous humor, cornea and iris-ciliary body were 0.42, 0.16 and 0.19 h, and T(1/2) were 0.1, 0.9 and 0.8 h respectively.</p><p><b>CONCLUSION</b>The method of determining 20S-ginsenoside Rg3 in rabbit eyes was established. The pharmacokinetics parameters were determined.</p>