Construction of Prognostic Prediction Model for Lung Squamous Cell Carcinoma Based on Cuproptosis-related LncRNAs / 肿瘤防治研究

Objective To develop a new risk scoring model based on cuproptosis-related lncRNAs (CRLs) to predict the prognosis of lung squamous cell carcinoma (LUSC). Methods Data were obtained mainly from TCGA and GTEx databases. Univariate Cox, Lasso, and multivariate Cox regression analyses were conducted to determine CRLs that affect the prognosis of LUSC and establish a risk scoring model. The ability of risk score characteristics to independently predict LUSC survival was compared with that of clinical characteristics by calculating the area under the ROC curve (AUC). Immune-related functions and immune checkpoint differences were compared between high- and low-risk groups. Results Nine CRLs were selected as independent prognostic lncRNAs for LUSC, and a risk scoring model was developed. Risk score was the influence factor for the prognosis of LUSC. The AUC values predicted by the risk score model for 1-, 3-, and 5-year survival rates of patients with LUSC were 0.710, 0.718, and 0.743, respectively. The high- and low-risk groups were partly statistically different in terms of immune-related functional assays and immune checkpoint assays (P < 0.05). Conclusion The risk scoring model developed based on nine CRLs could predict the prognosis and immune therapy response of patients with LUSC in clinical practice.

Analysis on the source of students and the way to understand the policy of public and non-public undergraduates majoring in preventive medicine in Shandong Province / 中华医学教育探索杂志

Objective:To explore the source of students and the way to understand the policy of public and non-public undergraduate students majoring in preventive medicine.Methods:Using cluster random sampling, a total of 205 students from the first batch of public funded undergraduates and the same batch of non-public funded undergraduates of preventive medicine from Batch 2020 of two medical colleges in Shandong Province were selected as research objects. Questionnaires were issued on the platform of Sojump to investigate the source of students and the way to understand the policy. SPSS 22.0 statistical software was used to analyze the data, and the relative number and composition ratio were used to describe the counting data. Chi-square test was used to compare the characteristics of students and the understanding degree of the policy between government-funded undergraduates and non-government-funded undergraduates. Multiple responses were used to analyze students' understanding of the policy.Results:There were significant differences in the gender ( χ2=10.29, P<0.001), place of household registration ( χ2=5.61, P=0.018), father's educational level ( χ2=9.78, P=0.044) and the way to understand the policy ( χ2=17.19, P<0.001) of the public and non-public funded undergraduates majoring in preventive medicine. And 88.4% of the students knew about the policy of public medical students through their teachers, classmates and family members. Conclusion:There are more female government-funded undergraduates in preventive medicine than male students, and more rural students than urban students, with the spreading way of from mouth to mouth as the main approach to know this policy.

Construction of Lung Adenocarcinoma Prognosis Model and Drug Sensitivity Analysis Based on Cuproptosis Related Genes / 中国肺癌杂志

BACKGROUND@#Lung cancer is one of the most common malignant tumors in the world, and the current lung cancer screening and treatment strategies are constantly improving, but its 5-year survival rate is still very low, which seriously endangers human health. Therefore, it is critical to explore new biomarkers to provide personalized treatment and improve the prognosis. Cuproptosis is a newly discovered type of cell death, which is due to the accumulation of excess copper ions in the cell, eventually leading to cell death, which has been suggested by studies to be closely related to the occurrence and development of lung adenocarcinoma (LUAD). Based on The Cancer Genome Atlas (TCGA) database, this study explored the association between cuproptosis-related genes (CRGs) and LUAD prognosis, established a prognostic risk model, and analyzed the interaction between CRGs and LUAD immune cell infiltration.@*METHODS@#The RNA-seq data of LUAD tissue and paracancerous or normal lung tissue were downloaded from the TCGA database; the RNA-seq data of normal lung tissue was downloaded from the Genotype-tissue Expression (GTEx) database, and the data of 462 lung adenocarcinoma cases were downloaded from the Gene Expression Omnibus repository (GEO) as verification. T the risk score model to assess prognosis was constructed by univariate Cox and Lasso-Cox regression analysis, and the predictive ability of the model was evaluated by receiver operating characteristic (ROC) curve and calibration curve. Immune-related and drug susceptibility analysis was further performed on high- and low-risk groups.@*RESULTS@#A total of 1656 CRGs and 1356 differentially expressed CRGs were obtained, and 13 CRGs were screened out based on univariate Cox and Lasso-Cox regression analysis to construct a prognostic risk model, and the area under the curves (AUCs) of ROC curves 1-, 3- and 5- year were 0.749, 0.740 and 0.689, respectively. Further study of immune-related functions and immune checkpoint differential analysis between high- and low-risk groups was done. High-risk groups were more sensitive to drugs such as Savolitinib, Palbociclib, and Cytarabine and were more likely to benefit from immunotherapy.@*CONCLUSIONS@#The risk model constructed based on 13 CRGs has good prognostic value, which can assist LUAD patients in individualized treatment, and provides an important theoretical basis for the treatment and prognosis of LUAD.

Design and effectiveness evaluation of blended learning in epidemiology course for nursing undergraduates / 中华医学教育探索杂志

Objective To investigate the application and effect of blended learning in epidemiology course.Methods The 83 nursing undergraduates in one class of grade 2016 were enrolled as experimental group and the 122 nursing undergraduates in another class were enrolled as control group.The undergraduates in the experimental group received blended learning in epidemiology course,and those in the control group received traditional teaching.An anonymous questionnaire survey was performed after the course ended,and the teaching effect was assessed based on the score of a non-standard answer examination and the degree of recognition of comprehensive ability cultivation.SPSS 18.0 was used for data analysis,and the t-test and the chi-square test were used for comparison of data between groups.Results The experimental group had a significantly higher total score than the control group [(85.55 ± 10.49) vs.(80.07 ± 9.47),t=3.897,P=0.000].Compared with the control group,the experimental group had a significantly higher degree of recognition of independent learning ability,cooperative ability,knowledge application,and problem-solving ability after blended learning (P＜0.05).Conclusion Blended learning can be applied in epidemiology course,and its teaching effect is accepted by students.

Application of problem-based learning in experimental teaching of medical statistics for medical undergraduates / 中华医学教育探索杂志

Objective To study on application of problem-based learning in experimental teaching of medical statistics for medical undergraduates and to improve the quality of experimental teaching in medical statistics. Methods 72 medical undergraduates were selected at random. They were devided into the PBL group and the control group were observed for a term. Results The comparison of a questionnaire showed a clear preference for PBL group over the control group. Most of the constituent ratios in the PBL group were higher than the control group, showing significant difference(P

Effect of Ischemia-Reperfusion Injury on Apoptosis of Pancreatic Cells in Rats with Acute Pancreatitis / 中国普外基础与临床杂志

Objective To investigate the effect of ischemia reperfusion (I/R) injury on apoptosis of pancreatic cells in rats with acute pancreatitis(AP). Methods Fifty four SD rats were randomized into 3 groups: pancreatitis group ( n =24), I/R injury group ( n =24) and control group ( n =6). The animal model of AP was induced by retrograde injection of 3% sodium taurocholate into biliopancreatic duct in rats. Pancreatic I/R was caused by blocking the inferior splenic artery and removing the clamp after AP induction. At 1 h, 3 h, 6 h and 12 h, groups of rats were sacrificed. A terminal deoxynucleotidyl transferase mediated dUTP biotion nick end labeling (TUNEL) was used to detect pancreatic apoptosis, and histological changes of the pancreas were observed. Results Pancreatic hemorrhage, necrosis were respectively observed in the pancreatitis rats at 6 h and the I/R injury rats at 1 h. Histological changes of the pancreatitis rats at 1 h and 3 h were only congestion and edema. Apoptoic acinar cells increased after AP induction, the peak respectively appeared at 6 h in the pancreatitis rats and at 3 h in the I/R injury rats. Compared with the pancreatitis rats, apoptosis index (AI) of the I/R injury rats was significantly higher at 1 h and 3 h ( P

The characteristics and tendency of unexpective death in the elderly of Jing'an District ,Shanghai in 1986～2003 / 中华老年医学杂志

Objective To know the characteristics and tendency of unexpective death in the elderly of Shanghai downtown area. Methods The data of the elderly unexpective death in Jingan District were divided into 4 groups. The causes and frequency of unexpective death were analyzed. Results The average rate of unexpective death in the elderly of Jingan District were from 208/ 100 000 per year in 1986～1990 to 11/ 100 000 per year in 2001～2003.It descended about half.Fall and suicide death rate descended about half as well. Since 1986, there was no descent tendency for traffic accident and poisoning death. Conclusions Unexpective death in elderly is preventable and needs to pay much concern by relative departments.

THE DISTRIBUTION OF INTRATHALAMIC VESSELS IN HUMAN BRAIN / 解剖学报

The course, distribution of the intrathalamic vessels on 8 sides of newborn brains were studied by means of the continuous artery-vein infusion, Spalteholz's clearing and Nissl's staining. The brains were all cut into the coronal sections of 500 ?m, 100?m and 30?m thickness in alternate and successive order. The main arteries supplying thalamic nuclei were: 1. The thalamoperforating artery; 2. The geniculothalamic artery; 3. The medial posterior choroidal artery; 4. The medial inferior pulvinar artery; 5. The lateral ventricular choroidal artery. The thalamic veins opened respectively into the internal cerebral vein, the veins of the lateral ventricle, the veins of the interpeduncular fossa and the basal vein. There were three patterns of course relation between thalamic arteries and veins. They were solitariness, accompanying and one vein surrounded by several arteries.

QUANTITATIVE STUDY ON THE CAPILLARIES OF THE HUMAN THALAMUS AND ITS ADJACENT STRUCTURES / 解剖学报

The brains from four newborns were used in this study. After continuous artery-vein injection with coloured materials, the brains were embedded in nitrocellulose. and coronal sections of 500?m, 100?m and 30?m in thickness were prepared in alternative and successive sequence. The 500?m sections were cleared in wintergreen oil and mounted, and the 100?m and 30?m sections were stained with thionin. The diameter and density of capillaries in thalamus and its adjacent structures were measured by means of Leitz MPV-Tasplus multifunction image analyser. All the data were dealt with variance and correlation analysis statistically. The capillary density of the thalamus and its adjacent structures varied obviously. The value in putamen, cellular layers of lateral geniculate body, anterior thalamic nucleus and subthalamic nucleus was the highest; while the value in dorsomedial nucleus, ventral lateral nucleus, pulvinar nucleus, ventral posterior lateral nucleus, lateral posterior nucleus, centromedian nucleus, medial geniculate body, ventral anterior nucleus and internal medullary lamina was the intermediate; however, the value in internal capsule and fibrous layers of lateral geniculate body was the lowest. The capillary diameter of ventral anterior nucleus and internal capsule was the widest; that of putamen and cellular layers of lateral geniculate body was the smallest; the rest was the intermediate. In the same measured structures, negtive correlation was shown between the capillary diameter and density.