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OBJECTIVE:To establish the gas chromatography (GC) fingerprint for the petroleum ether part of Areca peel and provide reference for the quality evaluation. METHODS:GC was conducted to establish the fingerprint for the petroleum ether part of Areca peel from 10 different areas,the cluster analysis and similarity of fingerprint data were conducted to study the similarity of GC fingerprint of Areca peel. RESULTS:There were totally 8 common peaks. All the medicine materials had the characteristics,but there were differences among the relative peak area. CONCLUSIONS:The GC fingerprint has high importance and precision and can be used for the quality control of Areca peel.
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Objective To establish a HPLC method for determining the related substances of valsartan and amlodipine tablets. Methods With octadecylsilane chemically bonded silica as the filler,the mixture of triethylamine(adjusting pH to 2.8 with phos-phoric acid)-methanol-acetonitrile was acted as the mobile phase by the gradient elution.The flow rate:1 .0 mL/min.The tempera-ture:40 ℃.The detection wavelength:238 nm.The injection volume:10μL.Results With octadecylsilane chemically bonded sili-ca as the filler,the mixture of triethylamine(adjusting pH to 2.8 with phosphoric acid)-methanol-acetonitrile was acted as the mo-bile phase by the gradient elution.The flow rate:1.0 mL/min.The temperature:40 ℃.The detection wavelength:238 nm.The injection volume:10μL.Conclusion This method is simple and accurate with good system suitability and can be used as the test method for the related substances of valsartan and amlodipine tablets.
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Objective To optimize the formulation of cyanidenon-?-cyclodextrin inclusion compound.Methods Cyanidenon-?-cyclodextrin inclusion compound was prepared by saturated water solution method.The effects of influence factors,such as ingredient proportion,inclusion time,inclusion temperature on inclusion rate,obtaining rate,and overall desirability were investigated by using Box-Behnken design and response surface method.The data were imitated using multi-linear equation and second-order polynomial equation.Results The latter was prior to the former considering from multiple correlation coefficients.Under the optimal conditions,inclusion rate and yield were 75% and 66%,respectively.Conclusion The optimized preparation technique for cyanidenon-?-cyclodextrin inclusion compound is stable,feasible,and has high inclusion rate.It can be used for the production of cyanidenon-?-cyclodextrin inclusion compound.
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Objective To improve the permeation of nasal mucosa by preparing the cholic acid-hydroxypropyl-?-cyclodextrin(HP-?-CD) and characterize the inclusion complex.Methods The inclusion complex was characterized by using phase solubility,scanning electron microscope(SEM),Fourier transform-infrared(FT-IR),differential scanning calorimetry(DSC),and superconducting NMR;In an effort to investigate the effect of HP?-CD on cholic acid,the studies of in vitro permeation of nasal mucosa was performed.Results Phase solubility curve was AL type,K=564.30 L/mol,and the characterization de-monstrates the formation of inclusion complex;The permeability coefficients and steady state fluxes through ovine and porcine nasal mucosa were enhanced by the formation of inclusion complex with HP-?-CD about 1.46 and 1.44 times of the uncomplexed cholic acid,respectively.Conclusion The inclusion complex is formed and it could increase the permeability of nasal mucosa by preparing the cholic acid-HP-?-CD.
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AIM: To optimize the preparation of cholic acid-hydroxypropy-?-cyclodextrin inclusion complex,and its phase solubility analysis. METHODS: Orthogonal test,including molar ratio,inclusion temperature,mixing time and liquid dropping rate of cholic acid-HP-?-CD,was adopted to screen the optimal preparation,based on the inclusion efficiency by HPLC method.The solubilization effect was evaluated by using phase solubility.(RESULTS): The optimal preparation consisted of the molar ratio of cholic acid-HP-?-CD was 1∶3,60 ℃ inclusion temperature,60 min mixing time,1.6 mL/min liquid dropping rate;A_L type of phase solubility curve,K=564.30 L/mol,and 11.81 times solubilization. CONCLUSION: The optimal preparation is stable,reasonable and practicable with the encapsulation efficiency of 97.1%.Inclusing cholic acid with HP-?-CD is feasible,and the solubilization effect is significant.