Proteomic profiling of Acinetobacter baumannii ATCC 19606 and Malaysian isolated clinical strain AB-13 using 2-dimensional gel electrophoresis

Aims@#Acinetobacter baumannii has been identified as one of the six most pathogenic bacteria that is the cause of most hospital bacterial infections according to Infectious Disease Society of America (IDSA). These nosocomial pathogens are notorious worldwide due to its ability in causing lethal infections among immunocompromised patients and its resistance to many strong antibiotics. This study aims to compare the expressed proteins of two A. baumannii strain, ATCC 19606 and a pathogenic clinically isolated strain known as AB-13. @*Methodology and results@#AB-13 clinically strain was isolated from the lower respiratory tract of a patient with pneumonia. In this study, the proteomic profile of both ATCC 19606 and AB-13 are produced using 2-dimensional gel electrophoresis. The total protein contents were extracted, quantified and separated using 2-DE with a pH range of 4-7 to acquire the proteomic profile for comparison. The final analytical gel was analysed using Delta2D software and among the 324 protein spots successfully resolved, 10 spots exhibited signs of differential expression with 7 spots found to be downregulated and 3 spots upregulated (p< 0.01). These differences could signify the evolution AB-13 has undergone as it acquires traits ultimately aiding in its survivability, antimicrobial resistance and pathogenicity within varied environments especially during infections.@*Conclusion, significance and impact of study@#These findings support the presence of variation in AB-13 from a proteomic perspective, highlighting the pathogen’s evolution improving survivability and pathogenicity, warranting in-depth exploration towards understanding A. baumannii virulence and pathogenicity.

A Study on the Health Economics of General Practitioners in Malaysia: Trends, Challenges and Moving Forward

Health care systems play a vital role in providing health services and in optimising the population’s health of each nation. The Malaysian health care system primarily consists of the public and private health services. One of the prominent private health care services offered in the General Practitioner’s (GP) Clinic. Despite the prominent role GPs play in the health care system in this country, little is known about their practices, the issues and challenges faced by GPs in this country. The objective of this study was to describe the current GP practice operations in Malaysia in terms of its general operations, financial expenditure and revenue, market competitiveness and laboratory services offered by the clinics. A cross-sectional survey design was used in this study. The study sample comprised of 1800 GPs throughout the West of Malaysia selected using convenient sampling technique. This study provides the general description of the GP operations in terms of the nature of business, operations hours, and number of patients, the third party administrator and managed care organisation linkages, financial expenditure, market competitiveness and laboratory services. The findings of the study reveal that the expenditure of managing GP services has increased over the years due to the changes in policies as well as the involvement of third party administrators in the healthcare system despite it playing an instrumental role in complimenting the healthcare services for the public at large.