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Mem. Inst. Oswaldo Cruz ; 85(4): 429-34, Oct.-Dec. 1990. ilus
Artículo en Inglés | LILACS | ID: lil-127780

RESUMEN

A rapid and simple technique for the purification of Toxoplasma gondii tachyzoites was developed. Highly purified parasites were obtained from the peritoneal exudates of infected mice by means of two consecutive discontinous sucrose gradients run at low speed (10,000xg, 30 min). Parasites obtained by this method conserved its biological activity. Hybridizations tudies with DNA from healthy mice and from purified tachyzoites preparations demonstrated that Toxoplasma gondii tachyzoites DNA could be obtained with better than 90 per cents purity. Preliminary studies with DNA endonucleases showed the presence in the tachyzoites genome of highly repetitives sequences


Asunto(s)
Animales , Ratones , ADN Protozoario/aislamiento & purificación , Genoma , Toxoplasma/genética , Southern Blotting , Centrifugación por Gradiente de Densidad , Secuencias Repetitivas de Ácidos Nucleicos , Toxoplasma/aislamiento & purificación
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