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1.
Beijing Da Xue Xue Bao ; (6): 665-672, 2019.
Artículo en Chino | WPRIM | ID: wpr-941867

RESUMEN

OBJECTIVE@#To investigate the impact of preoperative diagnostic ureteroscopy and biopsy (UB) on radical nephroureterectomy (RNU) and the prognosis of upper tract urothelial carcinoma (UTUC).@*METHODS@#The clinical data of UTUC patients receiving RNU between Jan. 2007 and Dec. 2016 were retrospectively collected. The median follow up time was 40 months. The operation time and blood loss of RNU were compared between UB group and non-UB group. Subgroup analyses were conducted according to the time interval between UB and RNU, and surgery methods of lower ureter. The linear regression model was used to adjust for other common factors that impacted operation time.@*RESULTS@#A total of 163 UTUC patients were included in the final analysis. For the lower ureter, open ureterectomies were performed in 91 patients (55.9%), while retroperitoneal laparoscopic ureterectomies were performed in 72 patients (44.1%). A total of 110 (67.5%) patients received preoperative UB. Compared with non-UB group, the average operation time of UB group was significantly longer [(252.5±79.8) min vs. (221.3±79.8) min, P=0.019], but no difference of blood loss was found (median, 50 mL vs. 50 mL, P=0.143). In subgroup analysis, the average operation time of RNU was significantly prolonged when RNU was performed after 1 week of UB (P=0.023). Meanwhile, the median blood loss of RNU increased significantly when it was done after 2 weeks of UB compared with non-UB group (100 mL vs. 50 mL, P=0.012). UB was also significantly prolonged the operation time of RNU in retroperitoneal laparoscopic ureterectomy group (P=0.012). In multivariable analysis, UB (P=0.049), ≥pT3 (P=0.039), pN+ (P=0.018) and ureterectomy method (P=0.005) were independent risk factors of prolonged operation time. The 3-year cancer specific survival (CSS) rate was 87.2% in our cohort. UB had no significant impact on cancer specific survival (P=0.435).@*CONCLUSION@#UB was an independent risk factor of prolonged RNU time, but did not significantly influence cancer specific survival of upper tract urothelial carcinoma patients.


Asunto(s)
Humanos , Biopsia , Carcinoma de Células Transicionales/diagnóstico por imagen , Nefrectomía , Nefroureterectomía , Estudios Retrospectivos , Uréter , Neoplasias Ureterales/diagnóstico por imagen , Ureteroscopía
2.
International Eye Science ; (12): 376-379, 2019.
Artículo en Chino | WPRIM | ID: wpr-719732

RESUMEN

@#AIM:To study the effect of biological keratoconjunctivitis combined with Ganciclovir Pellicles in patients with infectious keratitis and the effect on serum levels of inflammatory factors.<p>METHODS: In this study, 54 patients with 54 eyes were selected as the research object and divided into the observation group and the control group according to the random number table method. All patients were treated with bioartificial corneal transplantation. The patients in the observation group were treated with Ganciclovir eye drops, and the control group was treated with Aciclovir eye drops. The changes of LogMAR visual acuity, corneal transparency, serum inflammatory factors and cytokines were analyzed.<p>RESULTS: The rates of getting rid of blindness in the observation group and the study group were 93% and 89% respectively(<i>P</i>=0.642). The LogMAR visual acuity of two groups were significantly improved in 1wk and 6mo after operation compared with that before operation(<i>P</i><0.05). At 7d after operation, the levels of IL-6, TNF-α, INF- γ, SOD, NO and MDA in the two groups were significantly improved, and the improvement of the indexes in the observation group was better than that of the control group(<i>P</i><0.05). The rate of adverse reaction rate between two groups showed no significant difference(<i>P</i>=0.556).<p>CONCLUSION: Biological keratoconus combined with Ganciclovir has a good effect on viral keratitis, which can improve vision, inflammation and reduce the level of MDA.

3.
Yao Xue Xue Bao ; (12): 965-2016.
Artículo en Chino | WPRIM | ID: wpr-779264

RESUMEN

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed to elucidate the impurity profiles of paclitaxel and paclitaxel injections from different Chinese pharmaceutical companies. The fragmentation patterns for paclitaxel and the related impurities were analyzed and summarized. To remove the interference from auxiliary materials, such as hydrogenated castor oil, paclitaxel was dissolved in ethanol for acid, base, peroxide, and light induced forced degradation analysis, which could produce all the impurities exist in the paclitaxel injection. A total of 10 impurities were characterized, such as cephalomannine (1), 7-epi-10-deacetylpaclitaxel (2), 7-epipaclitaxel (3), baccatin Ⅲ (4), ethyl ester side chain (5), 7-epi-baccatin Ⅲ (6), 10-deacetylpaclitaxel (7), paclitaxel isomer (C3-C11 bridge) (8), paclitaxel isomer (9), and N-benzoyl-(2R, 3S)-3-phenylisoserine (10), respectively. Among them, compounds 1-3 could be introduced during manufacture processing. In the forced degradation studies, while acid induced degradation products included 3-7, base induced degradation could produce 2-7 and 10; while 7 is the main compound produced by hydrogen peroxide treatment, 4 compounds (3-5 and 7) were produced by high temperature environment and 5 compounds (2-5 and 9 which is the first reported) from intensity light exposure. Furthermore, 8 was the main impurity came from intensity light exposed paclitaxel powder. The results from this study provide an important reference in processing, optimization, quality control and evaluation of paclitaxel.

4.
Yao Xue Xue Bao ; (12): 856-862, 2012.
Artículo en Chino | WPRIM | ID: wpr-276232

RESUMEN

As nanomedicines are developing fast in both academic and market areas, building up suitable methods for nanomedicine analysis with proper techniques is an important subject, requiring further research. The techniques, which could be employed for grain size analysis of nanomedicines, were reviewed. Several key techniques were discussed with their principles, scope of applications, advantages and defects. Their applications to nanomedine analysis were discussed according to the properties of different nanomedicines, with the purpose of providing some suggestions for the control and administration of nanomedicines.


Asunto(s)
Sistemas de Liberación de Medicamentos , Luz , Microscopía Electrónica , Métodos , Microscopía de Sonda de Barrido , Métodos , Nanopartículas , Química , Clasificación , Tamaño de la Partícula , Dispersión de Radiación , Dispersión del Ángulo Pequeño , Espectrometría Raman , Métodos , Difracción de Rayos X , Métodos
5.
Chin. j. integr. med ; Chin. j. integr. med;(12): 93-99, 2012.
Artículo en Inglés | WPRIM | ID: wpr-289744

RESUMEN

To select the best interestingness measure appropriate for evaluating the correlation between Chinese medicine (CM) syndrome elements and symptoms, 60 objective interestingness measures were selected from different subjects. Firstly, a hypothesis for a good measure was proposed. Based on the hypothesis, an experiment was designed to evaluate the measures. The experiment was based on the clinical record database of past dynasties including 51 186 clinical cases. The selected data set in this study had 44 600 records. Cold and heat were selected as the experimental CM syndrome elements. Three indicators calculated according to the distances between two CM syndrome elements were obtained in the experiment and combined into one indicator. The Z score, ϕ-coefficient, and Kappa were selected from 60 measures after the experiment. The Z score and ϕ-coefficient were selected according to subjective interestingness. Finally, the ϕ-coefficient was selected as the best measure for its low computational complexity. The method introduced in this paper may be used in other similar territories.


Asunto(s)
Humanos , Temperatura Corporal , Enfermedad , Medicina Tradicional China , Modelos Biológicos , Reproducibilidad de los Resultados , Síndrome
6.
Yao Xue Xue Bao ; (12): 223-228, 2012.
Artículo en Chino | WPRIM | ID: wpr-323054

RESUMEN

The paper reports the systematic study on felodipine and its impurities in tablets, to improve its quality standards for the control of the related substances. HPLC-DAD, UPLC-MS, IR and NMR methods were used for the isolation of felodipine and its impurities in tablets, their identification and the zebrafish animal model was used for the analysis of the toxic impurities. In felodipine material and its tablets, three impurities are isolated and identified. They are impurity 1 [dimethyl 4-(2, 3-dichlorophenyl)-2, 6-dimethyl-1, 4-dihydropyridine-3, 5-dicarboxylate], impurity 2 [ethyl methyl 4-(2, 3-dichlorophenyl)-2, 6-dimethylpyridine-3, 5-dicarboxylate] and impurity 3 [diethyl 4-(2, 3-dichlorophenyl)-2, 6-dimethyl-1, 4-dihydropyridine-3, 5-dicarboxylate], separately. The result of zebrafish animal model analysis showed that the teratogenic effects of four compounds were: impurity 3 > or = felodipine > impurity 1 > impurity 2, lethal effects were as follows: impurity 2 = impurity 3 > felodipine > or = impurity 1. This study confirmed the toxicity of three impurities in felodipine. According to the results, the paper suggested the amendments to the standard of the medicine and provided the support to the control of impurities in the manufacturing process.


Asunto(s)
Animales , Anomalías Inducidas por Medicamentos , Antihipertensivos , Química , Toxicidad , Bloqueadores de los Canales de Calcio , Química , Toxicidad , Cromatografía Líquida de Alta Presión , Métodos , Contaminación de Medicamentos , Felodipino , Química , Toxicidad , Espectroscopía de Resonancia Magnética , Estructura Molecular , Preparaciones Farmacéuticas , Química , Control de Calidad , Espectrofotometría Infrarroja , Comprimidos , Espectrometría de Masas en Tándem , Pez Cebra
7.
Yao Xue Xue Bao ; (12): 347-352, 2010.
Artículo en Inglés | WPRIM | ID: wpr-250651

RESUMEN

An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.


Asunto(s)
1-Desoxinojirimicina , Acarbosa , Cromatografía Líquida de Alta Presión , Métodos , Cromatografía de Fase Inversa , Inhibidores de Glicósido Hidrolasas , Hipoglucemiantes , Química , Inositol , Espectrometría de Masa por Ionización de Electrospray , Métodos , Espectrofotometría Ultravioleta , alfa-Glucosidasas
8.
Artículo en Chino | WPRIM | ID: wpr-331705

RESUMEN

The researching thinking approach of "descending the dimension with the lowering rank, ascending the rank with the raising of nsion of dimension", which could exclude the intervention of non-crucial fact ors and reveal the complexity of syndrome as well, provides a feasible approach for syndrome standardization. A study was conducted by analyzing some syndromes and their correlated factors selected from the "Ancient Good-sized Medical Records Database" through unconditional logistic multivariate stepwise regression by SAS 6.12 universal statistical software to sieve the variable, and the mathematical mode of regression equation was obtained eventually, expressed as logit (p) = alpha + beta1X1 + beta2X2 + ... + betamXm. With that, the research of syndrome standardization was proceeded on the syndromes with diagnostic significance to provide references for standardization of syndrome differentiation. I


Asunto(s)
Humanos , Diagnóstico Diferencial , Modelos Logísticos , Medicina Tradicional China , Métodos , Estándares de Referencia , Estándares de Referencia , Proyectos de Investigación
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