RESUMEN
<p><b>OBJECTIVE</b>To establish an RP-HPLC method for determination of betulinic acid in Callicarpa macrophylla, a commonly used herbal in Yunnan.</p><p><b>METHOD</b>A Kromasil-C18 column (4.6 mm x 200 mm, 5 microm) and a mobile phase consisted of acetonitrile-0.1% phosphoric acid (63: 37) were used. The flow rate was 1.0 mL x min(-1) and the UV detector wavelength was 205 nm.</p><p><b>RESULT</b>Betulinic acid was well separated from other compounds in C. macrophylla. The content of betulinic acid in C. macrophylla from different origins showed apparent differences, the content of betulinic acid in C. macrophylla from Yunnan was the highest. The calibration curve was linear in the range of 0.016 6-0.332 mg x mL(-1) of betulinic acid with correlation coefficient 0.999 8. The average recovery of betulinic acid was 98.5%.</p><p><b>CONCLUSION</b>The method is simple, accurate and reproducible, and is suitable for the quality control of C. macrophylla.</p>
Asunto(s)
Calibración , Callicarpa , Química , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos , Química , Modelos Lineales , Control de Calidad , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , TriterpenosRESUMEN
Aristolochic acid I (AA-I) was absorbed and distributed quickly in vivo, the plasma concentration-time curve were fit with the open two-compartment model and one-compartment model, respectively. The elimination of AA-I has relationship with the dosage, the low dose group eliminates more quickly than the high dose group. The characters of pharmacokinetics of AA-I induce the cumulation of AA-I in vivo and the nephrotoxin to the kidney and other viscera.
Asunto(s)
Animales , Humanos , Administración Oral , Aristolochia , Química , Ácidos Aristolóquicos , Farmacocinética , Toxicidad , Relación Dosis-Respuesta a Droga , Inyecciones Intravenosas , Riñón , Metabolismo , Enfermedades Renales , Lactamas , Metabolismo , Toxicidad , Hígado , Metabolismo , Plantas Medicinales , Química , Distribución TisularRESUMEN
<p><b>AIM</b>To investigate the chemical constituents of the rhizome of Anemone raddeana Regel, so as to find new active compounds.</p><p><b>METHODS</b>The ethanol extracts of the rhizome of Anemone raddeana were obtained by silica column, HPLC. The structures of the compounds were elucidated by means of physico-chemical method and spectral analysis (IR, FAB-MS, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, HMQC, HMBC).</p><p><b>RESULTS</b>Nine compounds were isolated and identified as 27-hydroxyolean-12(13)-en-28-oic acid-3-0-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside (1), eleutheroside K (2), Oleanolic acid-3-O-alpha-L-rhamnopyranosyl-(1-->2)-[beta-D- glucopyranosyl-(1-->4)]-alpha-L-arabinopyranoside (3), betulin (4), betulic acid (5), acetyloleamolic acid (6), evonymitol (7), oleamolic acid (8) and diosgenin (9).</p><p><b>CONCLUSION</b>Compound 1 is a new compound, named raddeanoside 12. Compounds 3-7 were isolated from this plant for the first time.</p>