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Objective:To compile and evaluate the questionnaire of knowledge, belief and practice of perioperative nurses in the prevention of deep venous thrombosis (DVT) in order to provide a tool for further investigation of the status quo of nursing care in the prevention of DVT during perioperative period.Methods:Based on the theoretical model of knowledge, belief and practice, a questionnaire item pool was established by literature reading and semi-structured interviews, and an initial questionnaire was formed by Delphi expert inquiry. It was convenient to select 197 nurses engaged in surgery and operating room nursing in Qingdao Municipal Hospital Affiliated to Qingdao University to carry out the pre questionnaire, and to carry out the differentiation levels,relevance coefficient analysis and reliability and validity test were used to form a formal questionnaire.Results:After two rounds of Delphi expert inquiries, three items with lower assignment value and four items with modified contents were deleted to form the initial questionnaire. The positive coefficients of experts in two rounds of inquiry were 85.00% and 100.00%, respectively; the Kendall's harmonious coefficients of experts were 0.195 and 0.204 ( P<0.01); and the authoritative coefficients of experts were 0.836 and 0.849. Four items were deleted from 197 pre-survey questionnaires by discriminatory analysis, correlation coefficient analysis and reliability and validity test. The Item Level Validity (I-CVI) of the total questionnaire was 0.800-1.000, and the Scale Level Validity (S-CVI) was 0.825. In the structural validity analysis, the KMO value of each item was 0.824, the Bartlett spherical test value was 3170.304, P < 0.01. Twelve common factors with eigenvalues greater than 1 were extracted, and the cumulative variance contribution rate was 66.11%. The intrinsic consistency Cronbach a coefficient of the total questionnaire was 0.859, and the retest reliability was 0.706. Finally, a formal questionnaire with 40 items in 15 knowledge dimensions, 10 belief dimensions and 15 behavior dimensions was formed. Conclusion:The reliability and validity of the questionnaire are well evaluated, and it can be used as a tool for investigating the knowledge, belief and practice of perioperative nurses in preventing deep venous thrombosis.
RESUMEN
Objective@#To compile and evaluate the questionnaire of knowledge, belief and practice of perioperative nurses in the prevention of deep venous thrombosis (DVT) in order to provide a tool for further investigation of the status quo of nursing care in the prevention of DVT during perioperative period.@*Methods@#Based on the theoretical model of knowledge, belief and practice, a questionnaire item pool was established by literature reading and semi-structured interviews, and an initial questionnaire was formed by Delphi expert inquiry. It was convenient to select 197 nurses engaged in surgery and operating room nursing in Qingdao Municipal Hospital Affiliated to Qingdao University to carry out the pre questionnaire, and to carry out the differentiation levels,relevance coefficient analysis and reliability and validity test were used to form a formal questionnaire.@*Results@#After two rounds of Delphi expert inquiries, three items with lower assignment value and four items with modified contents were deleted to form the initial questionnaire. The positive coefficients of experts in two rounds of inquiry were 85.00% and 100.00%, respectively; the Kendall's harmonious coefficients of experts were 0.195 and 0.204 (P<0.01); and the authoritative coefficients of experts were 0.836 and 0.849. Four items were deleted from 197 pre-survey questionnaires by discriminatory analysis, correlation coefficient analysis and reliability and validity test. The Item Level Validity (I-CVI) of the total questionnaire was 0.800-1.000, and the Scale Level Validity (S-CVI) was 0.825. In the structural validity analysis, the KMO value of each item was 0.824, the Bartlett spherical test value was 3170.304, P < 0.01. Twelve common factors with eigenvalues greater than 1 were extracted, and the cumulative variance contribution rate was 66.11%. The intrinsic consistency Cronbach a coefficient of the total questionnaire was 0.859, and the retest reliability was 0.706. Finally, a formal questionnaire with 40 items in 15 knowledge dimensions, 10 belief dimensions and 15 behavior dimensions was formed.@*Conclusion@#The reliability and validity of the questionnaire are well evaluated, and it can be used as a tool for investigating the knowledge, belief and practice of perioperative nurses in preventing deep venous thrombosis.
RESUMEN
The porcine IL-18 gene was amplified from recombinant plasmid pGEM-IL-18 by PCR, then the pPIC9K-IL-18 of fusion expression vector was constructed by inserting IL-18 fragment,and was transformed to GS115 by electroporation, multi-copy recombinant strains were screened by G418. The expression of recombinant fusion protein was induced by methanol, SDS-PAGE was used to analyze expression product, fusion protein was purified by Sephadex G-100 column, bioactivity of IL-18 was measured by MTT assays. Experiment results show fusion protein of pIL-18 secreted by GS115,expression reaches the secretion peak of 160 mg/L at 72 h. We have expressed and purified successfully the recombinant pIL-18 with obvious biological activity in Pichia pastoris.