RESUMEN
A novel hemiacetal, citrinacetal (1) was isolated from a marine-derived fungus Penicillium citrinum by column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC. Its structure and stereochemistry was established on the basis of HR-ESI-MS, 1D and 2D NMR spectroscopic methods. The NMR spectrum showed this compound exists in solution as a mixture of two stereoisomers. The cytotoxic effect of compound 1 was evaluated in A-549, HL-60, HeLa, and K562 cancer cell lines. However, compound 1 only displayed weak cytotoxic activity on HL-60 cell, with IC50 value 77.4 micromol x L(-1).
Asunto(s)
Humanos , Acetales , Química , Farmacología , Antineoplásicos , Química , Farmacología , Cromatografía Líquida de Alta Presión , Células HL-60 , Células HeLa , Células K562 , Espectroscopía de Resonancia Magnética , Conformación Molecular , Estructura Molecular , Penicillium , Química , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
A new sesquiterpene hydroquinone (1) was isolated from a deep sea sediment derived fungus, Phialocephala sp.. Its structure and stereochemistry were established on the basis of spectroscopic data and optical rotation. This compound was tested for cytotoxicity against P388 (murine leukemia cell) and K562 (human leukemia cell) cell lines, and displayed strong cytotoxic effects with IC50 value of 0.16 and 0.05 micromol x L(-1), separately.
Asunto(s)
Animales , Humanos , Ratones , Antineoplásicos , Química , Farmacología , Ascomicetos , Química , Línea Celular Tumoral , Supervivencia Celular , Hidroquinonas , Química , Farmacología , Concentración 50 Inhibidora , Células K562 , Leucemia P388 , Patología , Estructura MolecularRESUMEN
In this study, 101 strains of bacteria were isolated from arct ic water and sediment samples. The methanol extracts of the fermented broth prod uced by these strains were screened in vitro for anti-tumor activity on mou se tsFT210 cells using the method of flow cytometry, and screened for antibacter ial activity by the method of paper disk diffusion. The result showed that one strain exhibited anti-tumor activity and eight strains had antibacterial activ ity. The stability of the antibacterial components produced by strain AR084 an d its optimum medium were also studied. The research indicated that arctic bac teria had potential application in pharmaceutics.