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Objective: To investigate the effect of endovascular intervention with Nordy-alginate microspheres (KMG) in treatment of rabbit VX2 hepatocellular carcinoma. Methods: Fifty rabbits with VX2 hepatocellular carcinoma were randomly divided into 5 groups (each n=10). The rabbits were then perfused of different drugs in the target vessels, which in group A was normal saline, in group B was Nordy, in group C was KMG, in group D were 5-fluorouracil+KMG and in Group E were Nordy-KMG. Enhanced CT scanning was performed 1 day before and 2 weeks after operation, and the volume and volume growth rate of tumors were compared among groups. Immunohistochemistry was used to detect the expression of vascular endothelial growth factor (VEGF) in tumor tissue, and the microvessel density (MVD) was counted. Results: There was no significant difference in the volume of tumors among 5 groups 1 day before operation (all P>0.05). Two weeks after operation, the volume and the volume growth rate of tumors in group A and B were higher than those in group C, D and E (both P<0.05). There were significant differences in the positive rate of VEGF and MVD among 5 groups (all P<0.01). The positive rate of VEGF and MVD in group E were lower than those in other groups (all P<0.05). Conclusion: Endovascular interventional therapy with Nordy-KMG is effective for VX2 hepatocellular carcinoma in rabbits.
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Objective To study the effect of azone,oleic acid,and their combination on the trans-dermal obsorption of baicalin.Methods Improved Franz-cells and HPLC were used for the transdermal study.The effect of azone,oleic acid,and their combination on the transdermal absorption of baicalin was studied using uniform design.Results The 24 h penetration amount of baicalin was obtained as 466.11 ?g/cm~2 and 77.34 ?g/cm~2 when the azone concentration was 10% or the oleic acid concentration was 14%,respectively.When using a combination of azone and oleic acid in the ratio of 10%∶0.5%,the 24 h penetration amount of baicalin was 641.88 ?g/cm~2 and the enhancement ratio of penetration was 146.69.Conclusion Both azone and oleic acid could improve the skin penetration amount of baicalin.Combinated application of azone and oleic acid could further enhance baicalin transdermal delivery rate.The maximum penetration amount of baicalin is obtained by using a combination of azone and oleic acid in the ratio of 10%∶0.5%.