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OBJECTIVE The expression of cancerous inhibitor of protein phosphatase 2A(CIP2A)in hypopharyngeal carcinoma FaDu cells(FaDu cells)was reduced by shRNA to understand its role in the occurrence and development of hypopharyngeal carcinoma.METHODS Specific shRNA sequence was designed,lentivirus was packaged and transfected into hypopharyngeal carcinoma FaDu cells,and CIP2A expression was specifically knocked down.The expression of CIP2A was detected by RT-PCR and Western blot.RESULTS 1.After shRNA knocked down CIP2A in FaDu cells,the CIP2A mRNA expression in the experimental group(CIP2A knocked down group)was significantly lower than that in the blank group,and the CIP2A protein expression in the experimental group was also significantly lower than that in the blank group.2.Cell cloning and CCK8 experiments showed that the cell proliferation ability of the experimental group was significantly decreased compared with that of the blank group(t=50.86,P<0.01;t=12.406,P<0.001);The results of cell scratch test showed that the transverse migration ability of the experimental group was significantly decreased compared with the blank group,and the longitudinal migration ability of the experimental group was significantly decreased compared with the blank group by Transwell test(t=40.038,P<0.01;t=12.247,P<0.001).CONCLUSION After knockdown CIP2A expression in hypopharyngeal carcinoma FaDu cells,the proliferation and migration ability of hypopharyngeal cancer cells decreased,suggesting that CIP2A is involved in regulating the biological behavior of hypopharyngeal cancer cells and can be used as a potential anticancer target.
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Objective To investigate the clinical characteristics,diagnosis and treatment of pelvic abscess complicated with septic shock.Methods The clinical symptoms,laboratory tests,surgical procedures,and prognosis were analyzed retrospectively for 9 patients with pelvic abscess complicated with septic shock.All the patients were treated in the Second Affiliated Hospital of Soochow University from August 2009 to March 2017.Results High fever and abdominal pain were reported in all the 9 patients,associated with hypotension,cardiac,pulmonary and renal dysfunction,and other symptoms.Eight patients were referred to ICU after early volume resuscitation,antimicrobial treatment,surgery or puncture.These 8 patients finally survived.The remaining one patient experienced multiple organ failure,disseminated intravascular coagulation,and extensive bleeding during treatment,and died finally.Conclusions Early diagnosis,adequate volume resuscitation,antimicrobial therapy,appropriate vasoactive drugs,and timely surgical removal of abscess are important for improving the survival of patients with pelvic abscess and septic shock.