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1.
Chinese Journal of Pathology ; (12): 95-100, 2013.
Artículo en Chino | WPRIM | ID: wpr-256245

RESUMEN

<p><b>OBJECTIVE</b>To retrospectively analyze the quantity and status of the tumor infiltrating regulatory T lymphocytes in breast cancer and the draining lymph nodes, and to elucidate the clinical pathologic significance.</p><p><b>METHODS</b>Seventy-four breast cancer samples with excised axillary lymph nodes were typed and staged histopathologically. The regulatory T lymphocytes were labeled by immunohistochemistry using EnVision method with the monoclonal antibodies against CD25 and Foxp3, and the immunophenotype was analyzed. In addition, the expression of IFN-γ, IL-10 and TGF-β1 mRNA in lymphocytes of lymph nodes draining the tumors was detected by in situ hybridization with the corresponding specific oligo nucleaic acid probes.</p><p><b>RESULTS</b>The number of CD25(+)Foxp3(+) T cells infiltrating the interstitium was much higher than that in the parenchymal tissue of the cancer. In the tumor draining lymph nodes, CD25(+) cells and Foxp3(+) cells were predominantly distributed in the paracortex with a proliferative pattern. TGF-β1, INF-γ and IL-10 mRNA positive cells showed a similar distribution pattern in the draining lymph nodes. Among the 39 cases with metastatic disease, the lymph nodes with metastases showed a much higher number of CD25(+)Foxp3(+) cells than that without metastases (23.5 vs 17.3 and 23.8 vs 15.5; P < 0.05). However, there was no difference in the density of Foxp3(+)CD25(+) cells in the draining lymph nodes between the death and survival groups (P > 0.05). Cytokine expression of TGF-β1, IL-10 and IFN-γ mRNA in the lymphocytes of draining lymph nodes in 24 cases showed that there were more IL-10 mRNA positive cells in the dead patients than that in the survived patients. A similar trend was observed for TGF-β1 mRNA positive cells but the difference was not statistically significant (P > 0.05). The expression rate of TGF-β1 and IL-10 mRNA in the draining lymph nodes was proportional to that of CD25(+) and Foxp3(+) cells (P < 0.05), and the expression of TGF-β1 positive cells was also proportional to that of IL-10 mRNA positive cells (P < 0.01). The expression of IFN-γ mRNA among these groups showed no significance (P > 0.05).</p><p><b>CONCLUSIONS</b>Regulatory T cells may play important roles in inhibiting the host antitumor immunity, and the presence of increased regulatory T cells and Th2-secreting cells in paracortex with a proliferative pattern in the tumor draining lymph nodes implies that the paracortical proliferation of draining lymph nodes may not reflect positive antitumor effects.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Persona de Mediana Edad , Neoplasias de la Mama , Metabolismo , Patología , Cirugía General , Estudios de Seguimiento , Factores de Transcripción Forkhead , Metabolismo , Hibridación in Situ , Interferón gamma , Genética , Metabolismo , Interleucina-10 , Genética , Metabolismo , Subunidad alfa del Receptor de Interleucina-2 , Metabolismo , Ganglios Linfáticos , Alergia e Inmunología , Metabolismo , Metástasis Linfática , Estadificación de Neoplasias , ARN Mensajero , Metabolismo , Estudios Retrospectivos , Tasa de Supervivencia , Linfocitos T Reguladores , Alergia e Inmunología , Metabolismo , Factor de Crecimiento Transformador beta1 , Genética , Metabolismo
2.
Chinese Journal of Pathology ; (12): 384-388, 2009.
Artículo en Chino | WPRIM | ID: wpr-249107

RESUMEN

<p><b>OBJECTIVE</b>To analyze retrospectively the quantity and activation status of the tumor infiltrating cytotoxic lymphocytes in breast cancer and the draining lymph nodes, and its relation to the clinical pathological significance.</p><p><b>METHODS</b>Seventy-four breast cancer samples with their corresponding axillary lymph nodes were histologically typed and staged. Cytotxic lymphocytes were analyzed by immunohistochemistry with the monoclonal antibodies against CD8, CD56, granzyme B and perforin.</p><p><b>RESULTS</b>The number of infiltrating CD8(+) T cells in the cancerous interstitial tissue were much higher than that in the tumor parenchyma. Compared with the metastatic tumor samples, the CD8(+) T cells were more intensive in the primary tumors (35.7 +/- 16.0 vs. 23.7 +/- 9.6). The tumor infiltrating CD8(+) T cells of patients with 5 years survivals were more than that of the dead cases in this follow-up series death (32.9 +/- 14.1 vs. 20.1 +/- 9.9). There was no significant difference of activated tumor infiltrating cytotoxic T cell analyzed by using the activation marker granzyme B(+) and there was also no significant correlation between the intensity of CD8(+), CD56(+) cells and the clinicopathological stages. However, percentages of the activated cytotoxic lymphocytes in Stage I groups were significantly higher than those in stage III and IV. Moreover, the number of perforin(+) cells was significantly less than that of granzyme B(+) cells, particularly in the cancerous tissue, indicating a dysfunctional status of tumor infiltrating cytotoxic lymphocytes.</p><p><b>CONCLUSIONS</b>Activated cytotoxic lymphocytes may play a significant role against the tumor progression and is associated with a favorable prognosis to some extent. However, a putative dysfunctional status of cytotoxic lymphocytes at tumor site may compromise the host immunity against cancer.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Persona de Mediana Edad , Axila , Neoplasias de la Mama , Metabolismo , Patología , Antígeno CD56 , Metabolismo , Antígenos CD8 , Metabolismo , Estudios de Seguimiento , Granzimas , Metabolismo , Inmunohistoquímica , Ganglios Linfáticos , Metabolismo , Patología , Metástasis Linfática , Linfocitos Infiltrantes de Tumor , Metabolismo , Patología , Estadificación de Neoplasias , Perforina , Metabolismo , Estudios Retrospectivos , Tasa de Supervivencia , Linfocitos T Citotóxicos , Metabolismo , Patología
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