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Journal of Biomedical Engineering ; (6): 584-587, 2013.
Artículo en Chino | WPRIM | ID: wpr-352205

RESUMEN

This study was aimed to construct transgenic mouse model with target for Runxl gene. Runxl cDNA of mice was amplified by PCR from pcDNA3. 1 Flag Runx1 FL vector and inserted into ptetO7-Asc-IRES-EGFP vector to form a recombinant vector, and then the recombinant vector was injected into fertilized egg by microinjection technology to get a transgenic mouse. The results of PCR and Southern blot indicated that the Runx1 transgenic mouse was constructed successfully, and this could provide an important tool for studying the function of Runxl gene in vivo.


Asunto(s)
Animales , Femenino , Masculino , Ratones , Secuencia de Bases , Subunidad alfa 2 del Factor de Unión al Sitio Principal , Genética , Vectores Genéticos , Genética , Ratones Endogámicos C57BL , Ratones Transgénicos , Genética , Microinyecciones , Datos de Secuencia Molecular , Recombinación Genética
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