Administration of a single chain variable fragments chimeric protein (SD) of ovalbumin epitopes internalizing receptor DEC-205 antibody inhibits food allergy in mice / 细胞与分子免疫学杂志

Objective To investigate the preventive therapeutic effect and possible mechanism of single chain variable fragments chimeric protein (SD) of ovalbumin epitopes internalizing receptor DEC-205 antibody on food allergy in mice. Methods Mice were randomly divided to five groups (control, PBS, scFv DEC 100 μg, SD 50 μg, SD 100 μg) and treated for 24 hours before OVA administration. After challenge, the serum level of OVA-specific IgE, IgG1, IgG2a and IL-4 were detected by ELISA. Infiltration of eosinophils and mast cells in the jejunum was observed by HE staining and toluidine blue staining respectively. The bone marrow of tibia and femur was isolated and cultured to obtain immature dendritic cells(BMDCs), which were further treated with LPS (10 ng/mL), TSLP (50 ng/mL), scFv DEC protein (1000 ng/mL) and SD protein (10,100,1000)ng/mL for 24 hours, and the IL-10 level of supernatant was assayed by ELISA. Results Compared with PBS group, the number of SD-treated mice with diarrhea was markedly reduced. The difference in rectal temperature and the levels of serum OVA-specific IgE, IgG1, IgG2a and IL-4 decreased significantly after prophylactic administration of SD; The number of eosinophils and mast cells in jejunum also decreased significantly while the IL-10 level in the supernatant of BMDCs increased significantly after SD intervention. Conclusion SD mitigates experimental FA response by fosters the immune tolerance property of dendritic cells.

Vagal Transient Receptor Potential Ankyrin 1 Mediates Stress-exacerbated Visceral Mechanonociception After Antral Cold Exposure

BACKGROUND/AIMS: Abdominal pain can be evoked or exacerbated after gastrointestinal cold stimulation in some patients with diarrhea-predominant irritable bowel syndrome (IBS-D), indicating a low temperature-induced sensitization of visceral perception. We investigated the role of vagal transient receptor potential ankyrin 1 (TRPA1, a cold-sensing ion channel) in cold-aggravated visceral mechanonociception in a stress-induced IBS animal model. METHODS: TRPA1 expression was examined in antral biopsies of healthy controls and IBS-D patients. Abdominal symptoms were assessed before and after warm or cold water intake. The visceromotor response (VMR) to colorectal distention (CRD) following intra-antral infusion of cold saline was measured in animals undergoing sham or chronic water avoidance stress. TRPA1 expression, extracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylation, and neuronal calcium influx in vagal afferents were assessed. RESULTS: Compared to healthy controls, IBS-D patients displayed elevated antral TRPA1 expression, which was associated with symptom scores after cold (4°C) water intake. Intra-antral infusion of cold saline increased VMR to CRD in naive rats, an effect dependent on vagal afferents. In stressed rats, this effect was greatly enhanced. Functional blockade and gene deletion of TRPA1 abolished the cold effect on visceral nociception. TRPA1 expression in vagal (but not spinal) afferents increased after stress. Moreover, the cold-induced, TRPA1-dependent ERK1/2 activation and calcium influx in nodose neurons were more robust in stressed rats. CONCLUSIONS: Stress-exaggerated visceral mechanonociception after antral cold exposure may involve up-regulation of TRPA1 expression and function on vagal afferents. Our findings reveal a novel mechanism for abnormal gastrointestinal cold sensing in IBS.

IgG4-related Disease in Digestive System / 胃肠病学

Immunoglobulin G4-related disease (IgG4-RD) is an immune-mediated fibroinflammatory disorder which can occur in different organs.It is characterized by massive IgG4 + lymphocytes and plasma cells infiltration,which leads to visceral enlargement,thickening or tubercle formation,and tends to form mass lesions.Most of the patients with IgG4-RD are associated with elevated levels of serum and tissue IgG4.This article reviewed the pathogenesis,clinical manifestations,diagnosis,treatment and prognosis of IgG4-RD in digestive system.

Protection of ovarian function for female breast cancer patients undergoing chemotherapy / 中华内分泌外科杂志

Breast cancer is one of the most common malignant tumors in females.Recent years,surgery,chemotherapy as well as other systemic therapy had greatly improved the prognosis of the patients.However,damage of ovarian function by chemotherapy lowered life quality,especially for young females.At present,there are several methods to protect the ovarian function of female patients undergoing chemotherapy,such as administration of a gonadotropin-releasing hormone (GnRH) analogs,ovarian cryopreservation,unfertilized ova cryopreservation,embryo cryopreservation,inhibitors of apoptosis,etc.Each method has its advantage,disadvantage and indications.Issues related to ovarian protection are reviewed here.

Effect of Dll4 at different concentrations on the biological functions of extravillous trophoblasts / 中国组织工程研究

BACKGROUND:Extravil ous trophoblasts exert a crucial effect on early pregnancy. When extravil ous trophoblasts function abnormal y, many pregnancy diseases can develop, such as preeclampsia, fetal growth restriction and placental implantation. OBJECTIVE:To investigate the effect of Dl 4, a ligand of Notch signaling family, on biological functions of extravil ous trophoblasts. METHODS:Human extravil ous trophoblast cel lines, HTR-8/SVneo, were cultured with human recombined protein Dl 4 of different concentrations (0, 0.125, 0.25, 0.5, 1.0 mg/L). Cel viability was tested by cel counting kit-8 assay. Transwel assay was applied to examine the changes in cel migration and invasion ability. RESULTS AND CONCLUSION:Dl 4 could significantly improve migration and invasion abilities of extravil ous trophoblasts, but had no effect on cel viability. In addition, Dl 4 regulated the invasion ability of extravil ous trophoblast in a dose-dependent manner. Above al , Dl 4 can play an important role in normal pregnancy by regulating the biological functions of extravil ous trophoblasts.