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Objective@#To compare the efficacy of Bracka method and Duckett method in the treatment of proximal hypospadias.@*Methods@#Forty patients with hypospadias were treated by 2 stages(Bracka), 42 patients treated by transverse preputial island flap (Duckett) from January 2014 to January 2016. Mean age at first stage surgery were (19.70±6.62) months and (20.33±5.03) months in Bracka group and Duckett group, respectively. There were 10 cases of proximal penile type, 25 cases of penoscrotal type, 5 cases of perineal type in group 1. There were 11 cases of proximal penile type, 27 cases of penoscrotal type, 4 cases of perineal type in group 2. There was no significant difference in age and hypospadias classification between the two groups(P>0.05). All operations were performed by the same doctor. Urethral plate reconstruction with preputial graft was performed in group 1; stage Ⅱ Duplay urethroplasty repair was carried out 6-8 months after stage Ⅰ.@*Results@#Urine tube was placed for 2 weeks after operation and followed up for 36-63 months(mean 47.6 months). After stage I repair, penile straightening and wide, smooth appearance of graft were confirmed. There was no fistula, split, urethral diverticulum or other complications, one case with urethral opening stenosis who was restored after urethral dilatation .After stage II repair, urethral fistula was noted in 3 cases(7.5%), stricture in 1 cases(2.5%). No other complications occurred . The total rate of complications was 10%(10/40). Urethral fistula was noted in 7 cases(16.7%), stricture in 3 cases(7.1%), penile head dehiscence in 3 cases(7.1%) and diverticulum in 1 case(2.4%) in group 2. The total rate of complications was 33.3%(14/42). The incidence of total complications between the two groups was statistically significant (P=0.011).@*Conclusions@#Bracka method can be used to treat proximal hypospadias. It has high safety and low incidence of complications.
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Objective To compare the efficacy of Bracka method and Duckett method in the treatment of proximal hypospadias.Methods Forty patients with hypospadias were treated by 2 stages (Bracka),42 patients treated by transverse preputial island flap (Duckett) from January 2014 to January 2016.Mean age at first stage surgery were (19.70 ±6.62) months and (20.33 ±5.03) months in Bracka group and Duckett group,respectively.There were 10 cases of proximal penile type,25 cases of penoscrotal type,5 cases of perineal type in group 1.There were 11 cases of proximal penile type,27 cases of penoscrotal type,4 cases of perineal type in group 2.There was no significant difference in age and hypospadias classification between the two groups (P ＞ 0.05).All operations were performed by the same doctor.Urethral plate reconstruction with preputial graft was performed in group 1;stage Ⅱ Duplay urethroplasty repair was carried out 6-8 months after stage Ⅰ.Results Urine tube was placed for 2 weeks after operation and followed up for 36-63 months (mean 47.6 months).After stage Ⅰ repair,penile straightening and wide,smooth appearance of graft were confirmed.There was no fistula,split,urethral diverticulum or other complications,one case with urethral opening stenosis who was restored after urethral dilatation.After stage Ⅱ repair,urethral fistula was noted in 3 cases(7.5％),stricture in 1 cases(2.5％).No other complications occurred.The total rate of complications was 10％ (10/40).Urethral fistula was noted in 7 cases(16.7％),stricture in 3 cases(7.1％),penile head dehiscence in 3 cases(7.1％) and diverticulum in 1 case (2.4％) in group 2.The total rate of complications was 33.3％ (14/42).The incidence of total complications between the two groups was statistically significant (P =0.011).Conclusions Bracka method can be used to treat proximal hypospadias.It has high safety and low incidence of complications.
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Objective To explore the feasibility of using composite scaffolds of rabbit oral epithelial cells and polycaprolactone (PCL) electrospun fibers for urethral repair. Methods The 25%PCL was prepared using a 5:1 by volume mixture of trichloromethane and anhydrous methanol, and PCL fiber tubular scaffolds were obtained by electrospinning. Rabbit oral mucosa epithelial cells (1.5 × 105) were implanted on the PCL scaffold. Subsequently, they were embedded in nude mice subcutaneous, explanted in 2 weeks. PCL fiber tubular scaffolds without rabbit oral mucosa epithelial cells were used as control. The complex urethral scaffolds were evaluated by immunofluorescence staining with cytokeratin antibody and HE staining. Results Compared with blank PCL group, the rabbit oral mucosa epithelial cell group showed a good cellularization. Rabbit oral mucosa epithelial cells formed a dense cell layer on the surface of PCL lumen, which suggested that rabbit oral mucosa epithelial cells can proliferate on the surface of PCL lumen. Conclusion Rabbit oral epithelial cells can be used as one of the seed cells for tissue engineered urethral scaffolds, and it is possible to construct tissue engineering substitute materials for urethral repair by rabbit oral epithelial cells combined with PCL.