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Objective:To establish a sensitive and fast HPLC method for determining cucurbitacin IIa in rat plasma and investigate the pharmacokinetic characteristics after intravenous administration. Methods: The analysis was performed on a Dikma Diamonsil C18 column (250 mm × 4. 6 mm, 5 μm) at 35℃ with the mobile phase of acetonitrile-water (45∶ 55, v/v) and the UV detection at 212 nm. The plasma samples were collected after intravenous administration at different time points and measured by HPLC. The pharmaco-kinetic parameters were calculated by DAS 2. 0 software. Results: The calibration plot of cucurbitacin IIa was linear over the range 0. 146-14. 060 μg · ml-1 . The range of method recovery was 99. 02%-104. 22% and that of extraction recovery was 84. 74%-86. 80%. The intra-and inter-day precision were both less than 5%. The stability met the requirements. The main pharmacokinetic pa-rameters were showed as follows:t1/2β(h):0.732 ±0.151, 0.681 ±0.055,0.667 ±0.064;Vd (L·kg-1):0.147 ±0.089, 0.131 ±0.095,0.153±0.047; Cl(L·h-1·kg-1): 0.287±0.031,0.304±0.063,0.318±0.029andAUC0→∞(mg·h·L-1):3. 646 ± 1. 124,4. 916 ± 1. 227,9. 385 ± 1. 419. Conclusion: The validated method is successfully applied in basic pharmacokinetic study in rat plasma after intravenous administration. The plasma concentration-time curves at three dosages are all fitted three-compart-ment model. Within the examined dose range, the pharmacokinetics of cucurbitacin IIa in rat is based on linear pharmacokinetics.
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Objective To provide a better reference for the quality control of Nujin Pian by establishing the method for the determination of paeonol in Nujin Pian by HPLC. Methods HPLC was used Agilent XDB C18 column (4.6 mm×150 mm, 4.6 μm), mobile phase was composed of methanol-water (45∶55). The flow rate was 1.0 mL/min with detection wavelength at 274 nm and column temperature at 40 ℃. Results The regression equation of paeonol was Y=7×107X-16 965, r 2=0.999 8 (n=6). The linear range of paeonol was 0.003 8-0.38 μg. The average recovery rate was 99.7%, and RSD was 1.56% (n=6). Conclusion The method is simple, fast and accurate with good reproducibility. It can provide reference for the quality control of Nujin Pian.
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OBJECTIVE:To study the quality standards of Compound kuqin ointment.METHODS:TLC method was used for the identification of alion,baicalin,menthol.The content of tetrahydropalmatine and baicalin were determined by HPLC.RESULTS:The identification method was specific.The linear range of tetrahydropalmatine was 0.131～3.275 ?g(r=0.999 9) with an average recovery of 102.40%(RSD=0.77%,n=6).The linear range of baicalin was 0.050 35～2.014 ?g (r=0.999 9)with an average recovery of 100.97%(RSD=1.42%,n=6).CONCLUSION:The method is simple,accurate and reproducible for the quality control of Compound kuqin ointment.
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OBJECTIVE:To establish the quality standard of Yanyan granules of concentrated sugar-free type. METHODS:Scrophularia ningpoensis,Menispermum dauricum,Radix et Rhizoma Glycyrrhizae in the granules were identified by TLC,and the content of linarin in Yanyan granules were determined by HPLC.RESULTS:The TLC spots were clear with high resolution.The linear range of linarin was 4.64～69.6 ?g(r=0.999 9) and the average recovery rate was 98.65%(RSD= 0.79%,n=6). CONCLUSION:The established standard is suitable for the quality control of the Yanyan granules of concentrated sugar-free type.