Risk Factors of Depression Screened by Two-Sample Mendelian Randomization Analysis: A Systematic Review / 生物医学与环境科学（英文）

OBJECTIVE@#This study explored the potentially modifiable factors for depression and major depressive disorder (MDD) from the MR-Base database and further evaluated the associations between drug targets with MDD.@*METHODS@#We analyzed two-sample of Mendelian randomization (2SMR) using genetic variant depression ( n = 113,154) and MDD ( n = 208,811) from Genome-Wide Association Studies (GWAS). Separate calculations were performed with modifiable risk factors from MR-Base for 1,001 genomes. The MR analysis was performed by screening drug targets with MDD in the DrugBank database to explore the therapeutic targets for MDD. Inverse variance weighted (IVW), fixed-effect inverse variance weighted (FE-IVW), MR-Egger, weighted median, and weighted mode were used for complementary calculation.@*RESULTS@#The potential causal relationship between modifiable risk factors and depression contained 459 results for depression and 424 for MDD. Also, the associations between drug targets and MDD showed that SLC6A4, GRIN2A, GRIN2C, SCN10A, and IL1B expression are associated with an increased risk of depression. In contrast, ADRB1, CHRNA3, HTR3A, GSTP1, and GABRG2 genes are candidate protective factors against depression.@*CONCLUSION@#This study identified the risk factors causally associated with depression and MDD, and estimated 10 drug targets with significant impact on MDD, providing essential information for formulating strategies to prevent and treat depression.

Establishment and practice of post management system for nursing staff in operating room / 中华护理杂志

According to the professional characteristics of the operating room,on the basis of basic principles of hierarchical management and post management of nursing department,taking clinical needs as guiding ideology,combined with the development needs of surgical departments,we developed the operating room nursing staff management model,and set up 23 jobs.We adjusted it accordingly in practice,and constantly optimized nurses' career plan by providing clinical,teaching,research,management and other multi-channel development direction,meanwhile provided better quality care for patients.

Establishment and practice of post management system for nursing staff in operating room / 中华护理杂志

According to the professional characteristics of the operating room,on the basis of basic principles of hierarchical management and post management of nursing department,taking clinical needs as guiding ideology,combined with the development needs of surgical departments,we developed the operating room nursing staff management model,and set up 23 jobs.We adjusted it accordingly in practice,and constantly optimized nurses' career plan by providing clinical,teaching,research,management and other multi-channel development direction,meanwhile provided better quality care for patients.

An analysis on genetic characterization of HA1 gene of influenza virus subtype H3N2 circulated from 2001 to 2006 in Liaoning local area / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To understand the HA1 genetic variation characterization of influenza virus subtype H3N2 circulated from 2001 to 2006 in Liaoning local area.</p><p><b>METHODS</b>Viral RNA was extracted and transcribed into cDNA by reverse transcriptase and amplified by PCR. The product of PCR was purified by QIAgen purification kits,and sequenced by ABI 3100avant. The sequence data were analyzed phylogenetically by Sequence software with epidemic records. Finally, the phylogenetic trees were drawn according to deduced amino acid sequences of influenza virus H3N2 from 2000 to 2006 in the NCBI database.</p><p><b>RESULTS</b>The seven HA1domain sequences of H3N2 influenza viruses circulated from 2001 to 2006 in Liaoning local area had been analyzed. Compared with WHO 2004-2006 H3N2 vaccine A/California/7/2004, 12 bases had changed, 4 positions had amino acid substitution in 62 * > E, 182 T > 1,224 S > A,225 C > Y. 224 and 225 are RBS (Receptor binding site). The homology is lower than 98%. Phylogenetic tree showed Liaoning H3N2 2006 strains and Zhejiang 2005 strains were similar to WHO Northern hemisphere winter 2006-2007 Vaccine A/Wisconsin/67/2005 (H3N2)-like virus and grouped together to form an independent cluster even though several bases were still different.</p><p><b>CONCLUSION</b>The HA1 domain of HA gene of influenza viruses (H3N2) isolated from 2001-2006 in Liaoning local area showed base mutation, amino acid sequence difference compared to A/California/7/2004 (2005-2006 vaccine), suggesting it might be the main cause leading to the spread of influenza. The sequence analysis showed Liaoning 2006 H3N2 strains were similar to those from Southern area which suggested that further surveullance should be conducted to monitor the virus mutation in circulation.</p>

Fucoidan: advances in the study of its anti-inflammatory and anti-oxidative effects / 药学学报

Fucoidan is a natural polysaccharide extracted from brown seaweeds, with a wide variety of biological features, especially the anti-inflammatory and anti-oxidative effects. Studies indicated that the anti-inflammatory effect of fucoidan related to its capacity to interact with the selectin or scavenger receptor on the cell membrane. Fucoidan can also inhibit the synthesis and release of reactive oxygen species (ROS), as well as promote its clearance, showing the anti-oxidative activity.

Influenza surveillance from 1999 to 2005 in Liaoning regions / 中华流行病学杂志

<p><b>OBJECTIVE</b>To investigate the prevalence and subtypes of influenza viruses in Liaoning regions from November 1999 to March 2005.</p><p><b>METHODS</b>Influenza virus was isolated by embryonated eggs together with cell culture and subtypes, identified by HI test.</p><p><b>RESULTS</b>During the study in 1999 - 2005, a total number of 2713 swab specimens were collected in different cities in Liaoning regions in which 188 strains were identified for influenza viruses with an average rate as 7.0%. A total number of 1466 swab specimens were collected by both Centers for Disease Control and Prevention in Dalian city and Liaoning province, and 167 strains were identified positive with an average rate of 11.4%. Influenza A3, A1 and B/Yamagata all appeared before March 2002 which were predominant strains. However, since then Influenza A1 has never appeared again in Liaoning regions and B showed some changes, from Yamagata to Victoria, the characteristics on the prevalence of influenza appeared only in the period of November to February.</p><p><b>CONCLUSION</b>It was meaningful to analyze the surveillance data of influenza in different years in Liaoning regions in order to better understand the characteristics of influenza and the shifting of subtype.</p>

Clinical evaluation of intraoperative block of abdomial aorta in sacral and pelvic tumors surgical operation / 中国癌症杂志

Purpose:To evaluate intraoperative block of abdomial aorta(BAA) in surgery of sacral and pelvic tumors as a useful adjuvant technique.Methods:Of 164 cases of pelvic tumor who underwent resections and hemisections, the procedures in 94 cases were non block of abdominal aorta(NBAA);in 109 cases of sacral tumors various extents of acrectomy was done,53 cases had NBAA,56 cases had BAA.Results:In the pelvic tumor group, the size of tumors for BAA were on the average bigger than NBAA by 0.8 cm, the operation was shortened by 2 h, the dose of hemorrhage decreased by 2 200 ml. The complications of operation were reduced by 6.3%, death rate reduced by 4.6%. In the sacral tumor group, the size of tumors for BAA were on the average bigger than NBAA by 0.7 cm, the operation was shortened by 1 h and 40 min, the dose of hemorrhage decrease by 1 600 ml. The complications of operations were reduced by 23%, death rate reduced by 7.5%.Conclusions:The adoption of BAA adjuvant technique can reduce the intraoperative hemorrhage,the death rate, and the complications of operation, while also shortening the surgical operating time, and is a valuable clinical technique. [

The enhancement effect of caffeine in cisplatin-induced apoptosis of osteosarcoma cells / 解放军医学杂志

Objective To explore the enhancement effect of caffeine in cisplatin-induced apoptosis of osteosarcoma cell line OS-U2. Methods The osteosarcoma cells were incubated with different concentrations of cisplatin (0.2, 2, 5, 10 and 20?g/L), caffeine (0.2, 2.0mmol/L) and caffeine + cisplatin for 24, 48 and 72 hours. The proliferation of OS-U2 cells was determined by MTT assay, the apoptotic levels were determined by flow cytometry (FCM), and the morphologic changes of apoptotic cells and the positive rates of apoptosis were determined by fluorescence microscopy. Results The proliferation of OS-U2 cells was inhibited, and the apoptotic level was increased when incubated with caffeine and/or cisplatin. There were dose- and time-effect relationships between the lethal effect of cisplatin on osteosarcoma cells and caffeine. Conclusion There is a remarkable enhancement effect of caffeine on cisplatin-induced apoptosis of osteosarcoma cell line OS-U2. It seems that the apoptosis-inducing activity of caffeine may enhance the lethal effect of cisplatin on osteosarcoma cells.