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Objective:To observe the effects of hydrogen on osteogenic capacity of human periodontal ligament cells(hPDLCs)stim-ulated with P.g-LPS.Methods:hPDLCs were cultured and divided into 4 groups:control(C)group,osteogenic induction(OI) group,OI +1 00 ng/ml LPS(OILPS)group and OIPLS +3%H2 (H2 OIPLS)group,and treated respectively.Alizarin red staining (ARS)was carried out 3 weeks after treatment.ALP and OC mRNA expression of the cells was examined by RT-PCR after 7-d treat-ment.Results:LPS decreased A value of ARS(P <0.01 ),ALP mRNA expression(P <0.001 )and OC mRNA expression(P <0.001 )of the cells.H2 increased the A value(P <0.05),ALP mRNA expression(P <0.01 )and OC mRNA(P <0.01 )of the cells treated by LPS.Conclusion:High concentration of P.g-LPS can inhibit osteogenic capacity of hPDLCs,while hydrogen can impair the P.g-LPS induced suppression of hPDLC's osteogenesis.
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Objective To evaluate the biocompatibility and antibacterial activities of the Ornidazole Slow-Release Membrane.Methods 1.The lower lips of 12 rats were sewed into 12 pockets and the pockets were immited with extracting solution of the ornidazole membrane, formaldehyde and normal saline respectively once per day.The specimens were examined histologically 7 days later.2.The dorsal muscles of 16 rats were implanted with the membranes or silk threads,and examined histologically 1 week and 2,4,6 weeks later respectively.3. The antibacterial activity against Streptococcus mutans and Fusobacterium nucleatum was observed on solid culture medium in vitro.Results The animal experiments showed the membranes were not irritative to the oral mucosa.It was found that the tissue reaction of the membranes was similar to that of the silk threads after implanted into dorsal muscles and the membranes had been degraded in the second week.And the membranes had effective antibacterial action against Streptococcus mutans and Fusobacterium nucleatum.Conclusion The Ornidazole Slow-Release Membrane possesses favorable biocompatibility and antibacterial activities.
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Objective To evaluate the potential of chlorhexidine(Chx)-thermosensitive hydrogel as a local drug delivery system for periodontal treatment.Methods The release profiles of Chx from CS-HTCC/GP thermosensitive hydrogel in vitro were investigated.Moreover,the antibacterial activity of S-HTCC/GP-0.1%Chx thermosensitive hydrogel to oral representative pathogens - Porphyromonas gingivalis(P.gingivalis),Prevotella intermedia(P.intermedia) and Actinobacillus actinorn ycetemcornitans(A.actinom ycetemcomitans) was determined by inhibitory zone measurement.Results Chlorhexidine(Chx) released in vitro over 18 hours from the CS-HTCC/GP thermosensitive hydrogel in artificial saliva pH 6.8 which more effectively retarded the release of Chx.Three periodontal pathogens were susceptible to Chx thermosensitive hydrogel with the inhibitory zone diameters over the range of 12～14mm.Conclusion All results indicated that Chx thermosensitive hydrogel can be used as a local drug delivery system for periodontal treatment.