Control of malaria outbreak due to Plasmodium vivax in Aswan governorate, Egypt

In 2014, after several years of maintaining zero malaria indigenous cases, Egypt had an outbreak of Plasmodium vivax: 21 confirmed cases during May-June 2014. In response to the outbreak, the Ministry of Health and Population [MoHP] launched an emergency response through early detection and prompt treatment of cases, vector control, public education and intersectoral collaboration. Twenty cases [95.2%] were residents of El-Sheikh Mostafa village, Edfu district, Aswan governorate, southern Egypt. All cases, consequent to the index case were identified through house-to-house surveillance visits. One P. falciparum-infected case was also identified in the same village. Treatment of all infected cases was initiated following laboratory confirmation. The MoHP's rapid response to and containment of the outbreak demonstrates the institutional capacity for detection and control of outbreaks which can occur after elimination

Impact of polystyrene beads as a mosquito control measure to supplement lymphatic filariasis elimination activities in Socotra Island, Yemen

Lymphatic filariasis [LF] is targeted for worldwide elimination. In Yemen, all mainland implementation units met the WHO criteria for stopping mass drug administration [MDA] after 5 rounds. However, in Socotra Island these criteria were not met. Our study evaluated the efficacy of applying expanded polystyrene beads [EPBs] on the Culex population and the effect on LF transmission. Human and mosquito surveys were conducted in 40 randomly selected households in Hadibo [capital of Socotra] before and after application of EPBs. The EPBs intervention resulted in a reduction in mosquito density of 80% and a 64.3% reduction in microfilaria prevalence. The majority of interviewed households [98%] thought EPBs considerably reduced the mosquito population. After the intervention all collected pools tested negative. Application of EPBs is an effective supplement to MDA for achieving the goal of LF elimination

Absence of lymphatic filariasis infection among secondary-school children in Oman

The endemicity status of lymphatic filariasis in Oman is uncertain, with only sporadic cases reported, mostly imported. Immunochromatographic card test surveys were carried out to assess the presence of circulating Wuchereria bancrofti antigenaemia as a marker for active infection in children from suspected high-risk areas of Oman [South Batinah and Dhofar]. Lot quality assurance sampling surveys were carried out on a minimum of 250 secondary-school children aged 17-18 years in each of 8 districts from February 2004 to March 2004. All tested students were negative for circulating W. bancrofti antigen. Based on these findings as well as previous data, Oman may possibly be classified as a nonendemic country, with no evidence of indigenous lymphatic filariasis transmission

retrospective hospital study of human cystic echinococcosis in Egypt

We performed a retrospective study to determine annual clinical incidence of human cystic echinococcosis [CE] in 14 Egyptian hospitals between January 1997 and December 1999. From 492 353 records examined, 133 [0.027%] new human CE cases were recorded. Of these, 50 [37.6%] were from Alexandria and Matrouh hospitals, 33 [24.8%] from Giza Chest Hospital and 50 from other regions. Matrouh governorate had the highest annual clinical incidence [1.34-2.60 per 100 000] followed by Giza governorate [0.80-1.16 per 100 000]. About a third of those affected were aged

Molecular characterization of Egyptian human and animal echinococcus granulosus isolates by Rapd-PCR technique

Five primers of known, but arbitrary nucleotide sequence [OPH-03, OPH-05, OPH-12, OPH-15 and OPH-18] were used to detect the genetic variability in the Egyptian human, camel and pig E. Granulosus isolates. OPH-03, OPH-05 and OPH-15 proved to be useful genetic markers of strain variation; while, OPH-12 and OPH-18 allowed the distinction at the genus level, i.e. diversified from Cysticercus tenuicollis. OPH-03 was the most effective, giving sharp distinct banding pattern and the least values of similarity coefficients. Some variations were detected within E. granulosus isolates from the same host. The level of heterogeneity was low in three of the human isolates, camel and pig strains. An individual variation was detectable within other three human isolates. Human and camel isolates were the most related pair, having similar patterns and the highest similarity coefficients. The study implied that human cases in Egypt are of the camel/dog strain and camels are important hosts for the transmission of human hydatidosis

Lymphatic filariasis in the Eastern Mediterranean Region: current status and prospects for elimination

Lymphatic filariasis [LF] represents a major public health problem in tropical and subtropical regions of the world. The disease is endemic or suspected in several countries of the Eastern Mediterranean Region. Recent advances in diagnosis and therapy led the World Health Assembly to pass a resolution in 1997 calling for "the elimination of lymphatic filariasis as a public health problem." The elimination strategy is based on rounds of mass drug administration of an annual single-dose of combined drug regimens for 5-6 consecutive years. Subsequent steps included formation of a Regional Programme Review Group to orient national LF control programmes towards the concept of elimination, provide advice, review each national plan of action and review annual reports. To date, Egypt and the Republic of Yemen have active national LF elimination programmes, however, elimination activities in the Republic of Yemen are still restricted to certain identified endemic regions. Other countries in the Region are on their way to verifying the situation and if LF is proved to be endemic, will start mapping endemic localities. This review sheds light on the status of LF elimination activities in the Region and highlights some of the major accomplishments

Filariasis elimination in Egypt: impact of low microfilaraemics as sources of infection for mosquitoes

The elimination strategy for lymphatic filariasis aims at reducing blood microfilaraemia to levels at which vector transmission cannot be sustained. We aimed to determine whether patients with pre-treatment low or ultra-low microfilaria [MF] counts could be a reservoir of infection after mass drug administration [MDA] with a combined regimen. Laboratory-reared mosquitoes were fed on 30 volunteers after 2 rounds of MDA. Microfilaria uptake, infectivity rates and number of Wuchereria bancrofti L3 per mosquito were assessed. One year after MDA-1, 6 subjects transmitted MF, but up to 9 months after MDA-2 transmission failed. Six months after MDA-2 > 90% had clear MF smears and either failed to transmit MF or transmitted MF that did not develop to L3. We conclude that the transmission cycle is seriously weakened after MDA-2

longitudinal study of Schistosome intermediate host snail populations and their trematode infection in certain areas of Egypt

The seasonal variation of Biomphalaria alexandrina and Bulinus truncatus populations and their infection rates with schistosome and other trematode cercariae were studied longitudinally in four water courses located in Giza and Fayoum governorates. The abundance of both species varied from year to year and according to the type of habitat. The mean prevalence of Schistosoma mansoni in Biomphalaria was 0.29% and that of S. Haematobium in Bulinus was 1.36%. The seasonal variations of age structure of the two vector snails were monitored throughout the survey period. Infection rates with schistosome and other trematodes among Bulinus and Biomphalaria increased with the increase in snail size. Data suggested the occurrence of an antagonistic interaction between schistosome and non- human cercariae, especially echinostome in infected snails

Studies on the molluscicidal and larvicidal properties of Solanum nigrum L. leaves ethanol extract

Toxicological studies on three ethanol extract preparations of Solanum nigrum L. leaves were made on Biomphalaria alexandrina. Extract [A], made by soaking leaves powder over night in cold 70% ethanol, has the highest activity, [LC[50] 3.37mg/L within 24 hr]. This extract also showed larvicidal activity against larvae of two mosquito species, Aedes caspius and Culex pipiens, [LC[50] 51.29 and 125.89 mg/L within 24 hr, and 21.38 and 38.11mg/L within 48 hr, respectively]. Sunlight, pH, and turbidity did not affect the activity of this extract, but the molluscicidal activity seems to be correlated with the increase of temperature. The concentrated extract [1000mg/L] can be stored at room temperature for six months without any change in its activity, but diluted solutions of this extract lost their activity after four weeks

Infection of two lymnaeid snails with fasciola gigantica in Giza, a field study

Population density of Lymnaea natalensis and L. columella and their natural infection with Fasciola gigantica were followed in two water bodies in Abou-Rawash area, Giza Governorate over two years. L. natalensis snails were more abundant during December- February, while L. columella snails had high density during autumn. According to the snail size, it was found that the reproduction of the two vector snails occurred in summer and early autumn. Fasciola was presented in 0.6 and 2.0% of the examined L. natalensis and L. columella snails, respectively. A high prevalence of F. gigantica in L. columella snails occurred in summer and early autumn, while their presence in L. natalensis was irregular. Naturally infected Lymnaea snails, with Fasciola, are usually of large size [>10 mm] of a given population sample

Selection of susceptible lines of biomphalaria alexandria and bulinus tuncatus snails to S mansoni and S haematobium infection

Selection of progenies from infected B. Alexandrina and B. truncatus snails resulted in higher infection rates. This observation was consistent when the source of micardia were from either infected human or hamsters. Infection rate of B. Alexandrina increased up to 88.2% while B. truncatus did not. Compatibility between the parasites and their intermediate hosts were also studied