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The Korean Journal of Parasitology ; : 197-201, 2013.
Artículo en Inglés | WPRIM | ID: wpr-103954

RESUMEN

A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.


Asunto(s)
Animales , China , Análisis por Conglomerados , Cisticercosis/parasitología , ADN de Helmintos/química , ADN Mitocondrial/química , Complejo IV de Transporte de Electrones/genética , Variación Genética , Enfermedades de las Cabras/parasitología , Cabras , Filogenia , Reacción en Cadena de la Polimerasa , Subunidades de Proteína/genética , Análisis de Secuencia de ADN , Ovinos , Enfermedades de las Ovejas/parasitología , Taenia/clasificación
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