Looks can be deceiving: A case of placental mesenchymal dysplasia with concomitant cytomegalovirus infection / Philippine Journal of Obstetrics and Gynecology

Pregnancy with placental mesenchymal dysplasia (PMD), with concomitant cytomegalovirus (CMV) infection, and with coexistent normal viable fetus is very rare. An 18 year old, Gravida 2 Para 0 (0-0-1-0), was referred to our institution at 24 weeks age of gestation, with impression of molar pregnancy with a live twin fetus. Subsequent ultrasound revealed placentomegaly with placental features similar to hydatidiform mole. Her pregnancy was complicated with intrauterine growth restriction (IUGR) and oligohydramnios. TORCH panel at 25 weeks age of gestation revealed positive for CMV IgG. She had normal serum ?-HCG, with elevated maternal serum alpha feto protein (msAFP). A caesarean section was done at 37 weeks age of gestation for placenta previa marginalis in haemorrhage. She delivered a live baby girl, APGAR score of 9,9, maturity testing of 37 weeks, small for gestational age. The baby was also positive for CMV IgG. Gross examination of the placenta revealed tortuous vessels and multiple cystic spaces. Histopatho-logically, there were hydropic stem villi with no trophoblastic proliferation. Immunohistochemically, placental mesenchymal dysplasia was confirmed.

Antígenos liofilizados de Babesia bovis em ELISA para detecçäo de anticorpos / Assessment of lyophilised antigens in ELISA for Babesia bovis antibody detection

Uma preparaçäo antigênica bruta de Babesia bovis, preparada a partir de cultivo celular, foi liofilizada e utilizada em um teste de ELISA para detecçäo de anticorpos. O valor discriminante foi calculado pela média de amostras de soros negativos acrescida de dois desvios padröes. As duas séries de valores obtidas com antígeno liofilizado e antígeno congelado mostraram alta correlaçäo, indicando que a liofilizaçäo pode se constituir em uma alternativa prática para preservaçäo de antígenos de B. bovis para uso em ELISA