Purification and characterization of tubulin from mung bean ( Vignaradiata).

Tubulin has been purified from mung bean seedling by Zn2+-induced polymerization. Both a- and β-subunits of mung bean tubulin are different from those of brain tubulin in electrophoretic mobility, colchicine binding and peptide map. Heterogeneity of mung bean tubulin has also been documented suggesting diversification of tubulin despite its conserved nature in general.