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1.
Indian J Exp Biol ; 2013 Feb; 51(2): 107-115
Artículo en Inglés | IMSEAR | ID: sea-147573

RESUMEN

Skin stem cells are very important in cosmetics, pharmacological and regenerative medicine and burn cases. Foreskin samples surgically removed after circumcision from boys below 7 years were collected and primary epidermal cells were prepared by enzymatic and mechanical tituration method. Selecting CD133 (prominin-1) multipotent stem cell marker, enriched stem cells were analyzed by MACS using CD133 antibodies conjugated with magnetic beads. CD133 positive and negative cells with specific skin stem cells markers like - CD34 (Universal stem cells marker), CD29 (integrin beta-1) and CD49f (integrin alpha-6) immunophenotypes were screened and sorted in flowcytometer. Further the expression of four embryonic genes or transcription factors of pluripotent stem cells were analyzed for pluripotent character of sorted cells. It was found that skin stem cell markers associated with CD133 cells, differentially expressed CD34, CD29 and CD49f immunophenotyes on both positive and negative CD133 cells in FACS analysis. The embryonic stem cell markers (induced pluripotent stem cell markers) like Oct4, SOX2, Notch-2 and K19 genes were expressed in CD133 positive epidermal cells. It is therefore evident that foreskin derived epidermal stem cells showed pluripotent or multipotent nature. This finding opens up avenues for new uses of these stem cells for direct cell seeding in wound healing, surgical suturing and drug screening.


Asunto(s)
Antígenos CD/metabolismo , Biomarcadores/metabolismo , Linaje de la Célula/genética , Separación Celular , Supervivencia Celular/genética , Niño , Epidermis/citología , Citometría de Flujo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Glicoproteínas/metabolismo , Humanos , Inmunofenotipificación , Masculino , Péptidos/metabolismo , Células Madre Pluripotentes/citología , Células Madre Pluripotentes/metabolismo , Propidio/metabolismo , Piel/citología , Coloración y Etiquetado
2.
Artículo en Inglés | IMSEAR | ID: sea-125120

RESUMEN

Abdominal cocoon, a rare condition in which the small bowel is encased in a membrane, resembles peritoneal fibrsis. There are only 16 case reports of this condition in the world literature. A 15-year-old girl presented with recurrent abdominal pain due to distal small bowel obstruction. Abdominal X-ray showed multiple air fluid levels like small intestinal obstruction. Contrast study revealed segregation of the small bowel loops with a dilatedproximal small intestine and gradual narrowing of the distal ileum with the obstruction; the mucosal pattern was preserved till the distal ileum. Contrast was not flowing into the colon. At laparotomy, the small intestine was seen to be encased and coiled up in a membrane. The membrane was excised, resulting in release of the obstruction.


Asunto(s)
Adolescente , Sulfato de Bario/diagnóstico , Medios de Contraste , Diagnóstico Diferencial , Femenino , Fibrosis , Humanos , Obstrucción Intestinal/etiología , Intestino Delgado/diagnóstico por imagen , Peritoneo/patología
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