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Asian Pacific Journal of Tropical Medicine ; (12): 517-520, 2014.
Artículo en Inglés | WPRIM | ID: wpr-820690

RESUMEN

OBJECTIVE@#To identify the genotypes of prevalent Cryptosporidia in broiler chickens in Lorestan province, Iran.@*METHODS@#A total of 1 000 fecal and 1 000 trachea samples were collected from chickens. Smears from both fecal and tracheal samples were stained with modified Ziehl-Neelsen method and nested PCR-RFLP according to amplification of 18S rRNA gene using Ssp I and Vsp I restriction enzymes and DNA sequencing.@*RESULTS@#From the examined chickens 0.7% was positive for Cryptosporidium. Infection was present in 0.5% fecal samples and also in 0.5% trachea. Only 0.3% of simultaneous infections in fecal and tracheal samples were observed. Nested PCR of our isolates demonstrated Cryptosporidium baileyi.@*CONCLUSIONS@#In our work, low rate of Cryptosporidium baileyi infection was detected, but in critical situations and our poor management circumstances, cryptosporidiosis occurs in serious feature especially in immune suppressed individuals.


Asunto(s)
Animales , Pollos , Parasitología , Criptosporidiosis , Epidemiología , Parasitología , Cryptosporidium , Genética , ADN Protozoario , Genética , Heces , Parasitología , Irán , Reacción en Cadena de la Polimerasa , Enfermedades de las Aves de Corral , Epidemiología , Parasitología , Prevalencia , Tráquea , Parasitología
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