RESUMEN
Objective: The aim of this study was to evaluate the fetal mouse lung tissue co-culture on in vitro maturation [IVM] of mouse immature oocytes
Materials and Methods: In this experimental study, germinal vesicle [GV] oocytes from ovaries of a group of 25 female mice, 6-8 weeks of age, were dissected after being stimulated by 7.5 IU pregnant mare serum gonadotropin [PMSG] through an intraperitoneal [IP] injection. The fetal lung tissues were then prepared and cultured individually. A total number of 300 oocytes were cultured in the following three groups for 24 hours: control group [n=100] containing only base medium, group I [n=100] containing base medium co-cultured with 11.5- to 12.5-day old fetal mouse lung tissues, and group II [n=100] containing base medium co-cultured with 12.5- to 13.5-day old fetal mouse lung tissues. The proportion of GV and metaphase ? [MI] oocytes matured into M?? oocytes were compared among the three groups using analysis of variance [ANOVA]. Correlation test were also used to evaluate the successful rate of IVM oocytes
Results: The proportions of GV oocytes reaching M?? stage were 46, 65, and 56%, in control, I and II groups, respectively [P<0.05]. The percentage of the oocytes remaining at the GV stage were higher in control group as compared with two treatment groups [P<0.05]
Conclusion: This study indicated that fetal mouse lung tissue co-culture method increased the percentage of GV oocytes reaching MII stage