RESUMEN
Background: The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis
Objective: In this study two endometriosis models at the morphological and molecular levels was evaluated and compared
Materials and Methods: The human endometrial tissues were cut into small fragments then they were randomly considered for transplantation into gamma irradiated mice as model A; or they were isolated and cultured up to fourth passages. 2×106 cultured stromal cells were transplanted into ? irradiated mice subcutaneously as model B. twenty days later the ectopic tissues in both models were studied morphologically by Periodic acid-Schiff and hematoxylin and eosin staining. The expression of osteopontin [OPN] and matrix metalloproteinase 2 [MMP2] genes were also assessed using real time RT-PCR. 17-beta estradiol levels of mice sera were compared before and after transplantation
Results: The endometrial like glands and stromal cells were formed in the implanted subcutaneous tissue of both endometriosis models. The gland sections per cubic millimeter, the expression of OPN and MMP2 genes and the level of 17-beta estradiol were higher in model B than model A [p=0.03]
Conclusion: Our observation demonstrated that endometrial mesenchymal stromal cells showed more efficiency to establish endometriosis model than human endometrial tissue fragments
RESUMEN
Objective: Endometriosis is a common disease in which the endometrial stroma and glands grow abnormally outside the uterine cavity. The establishment of animal models can be effective for determining the etiology of endometriosis. In this study we compare two endometriosis models using endometrial fragments and isolated cultured endometrial cells
Methods: We obtained endometrial tissues that were in the proliferative or secretory phases from women who underwent hysteroscopies for benign reasons at Imam Khomeini Hospital [Tehran]. Following confirmation of the tissue's normality, we cut the tissues into 2 mm cube pieces. The remainder of endometrial tissues were used for isolation and cultured to the fourth passage. In the first model of endometriosis, the endometrial tissue fragments and in the second model, 2×106 isolated endometrial cells were subcutaneously transplanted into gamma irradiated mice. The mice were kept under controlled, sterile conditions for 20 days. The mice sera were collected before and after transplantation for assessment of 17beta estradiol. The ectopic tissues in both models were assessed for morphological staining using hematoxylin and eosin, as well as periodic acid Schiff [PAS] for gland secretion. The gland sections per mm2 were analyzed
Results: At 20 days after tissue transplantation, we observed endometrial-like glands in the subcutaneous tissue of both endometriosis models. The number of gland sections was 57.55 +/- 17.18/mm2 for the first model and 271.57 +/- 77.98/mm2 for the second model. This result was significantly higher in the second model when compared to the first model. Gland secretion was positive for PAS. The level of 17beta estradiol was higher in both models compared to the control group. This level was significantly higher in the second model compared to the first [P
Conclusion: The endometriosis model that used cultured endometrial cells showed more efficiency in morphology, gland formation and level of17beta estradiol