RESUMEN
In vivo treatment of intestinal brush border membrane vesicles with solubilized insecticidal crystal proteins (ICP) from the two strains of B. thuringiensis var. israelensis (VCRC B17 and VCRC MB24) caused no adverse effect on gamma glutamyl transpeptidase, Na+K+ATPase, sucrase and lactase enzymes. But, exposure of membrane vesicles to solubilized ICP's in vitro, lead to significant reduction in the activity of Na+K+ATPase, sucrase and lactase enzymes.
Asunto(s)
Animales , Bacillus thuringiensis , Proteínas Bacterianas/farmacología , Toxinas Bacterianas/farmacología , Endotoxinas/farmacología , Proteínas Hemolisinas , Insecticidas/farmacología , Intestinos/efectos de los fármacos , Masculino , Microvellosidades/efectos de los fármacos , Control Biológico de Vectores , RatasRESUMEN
Effect of intact and alkali solubilized insecticidal crystal protein (ICP) preparations from a mutant strain of B. thuringiensis var. israelensis (VCRC MB24) and the wild type strain (VCRC B17) in vitro on human erythrocytes with respect to lipid peroxidation, osmofragility and membrane bound enzymes was determined. The alkali solubilized ICPs of both B. thuringiensis strains caused increased lipid peroxidation, decreased resistance to hypotonic lysis and reduction in the activity of membrane bound enzymes. On the contrary, the intact ICPs did not produce any such adverse effect on RBCs under the same experimental conditions. It is suggested that the ICPs are safe when they are intact when compared with solubilized ones.