Characterization of specific trichomonas vaginalis target antigens from different isolates by immuno-blotting against hyperimmune rabbit serum

The SDS-PAGE and immunoblot methods were used to identify Trichomonas vaginalis specific target antigen[s]. Ten T. vaginalis isolates, cultured on TYM media, were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis [SDS-PAGE], revealed 34 distinct bands. Immunoblotting against a hyperimmune rabbit serum, showed 20 reactive bands ranging from 14-205 kDa. There was isolate to isolate variability among 8 isolates, while 2 isolates [6, 7] showed a similar antigenic patterns. Imunoblotting of the isolates showed a total of 20 molecular weight bands, 80% of isolates gave positive immunologic reactions above 100 kDa, while below 100 Kda all the isolates recognized the different molecular weight bands. Out of 20 reacting bands, 5 main bands were detected, 29, 66, 84, 95 and 115 kDa gave positive percent of 60, 60, 60, 90 and 80% among the 10 isolates respectively

Growth patterns and antigenic analysis of Egyptian trichomonas vaginalis isolates

In this study, the vaginal washouts from symptomatic and asymptomatic patients were examined by wet mount examination and culture on modified TYM medium. Among the 320 examined cases, 10 were positive for T. vaginalis trophozoites by wet mount examination and culture. Modified TYM medium proved to be very satisfactory for the isolation as well as the maintenance of the ten T. vaginalis isolates. The comparison between the growth patterns of all isolates, by counting the number of viable organisms every 24 hours for 7 days, showed that there is a wide variability in the growth characteristics, as regards lengths of log phase, growth peaks reached, generation times, division rate and number of divisions. The antigenic differentiation of the ten T. vaginalis isolates through sodium dodecyl sulfate- polyacrylamide gel electrophoresis [SDS-PAGE] demonstrated a total of 34 bands using 10% resolution gel. The bands ranged in molecular weight from 12-189 kDa. Most of the bands were common among several isolates, while isolate 2 appeared different than other isolates with two characteristic bands, one at 136 kDa and the other at 25 kDa. Also, isolates 4 and 8 had characteristic bands at 163 kDa and 189 kDa, respectively