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Braz. j. med. biol. res ; 49(4): e4324, 2016. tab, graf
Artículo en Inglés | LILACS | ID: biblio-951663

RESUMEN

The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.


Asunto(s)
Humanos , Ligamento Periodontal/efectos de los fármacos , Interleucina-10/farmacología , Ligando RANK/metabolismo , Osteoprotegerina/metabolismo , Fibroblastos/efectos de los fármacos , Glucosa/farmacología , Ligamento Periodontal/citología , Factores de Tiempo , ARN Mensajero/análisis , Regulación hacia Abajo , Regulación hacia Arriba , Células Cultivadas , Western Blotting , Análisis de Varianza , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Fibroblastos/metabolismo
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