RESUMEN
Statement of the Problem: Oral squamous cell carcinoma is the most common oral malignancy. Toll-like receptor [TLR] activation led to alterations in the levels of mRNA encoding the TLR accountable for recognizing the inducing agonist and cross-regulation of other TLR
Purpose: The purpose of this study is determination of mitogen-associated protein kinase [MAPK] activation in human immortalized oral epithelial cell [HIOEC] line via up regulating of TLR7
Materials and Method: expression of TLR7 was measured in HIOEC and normal cells by quantitative real-time polymerase chain reaction [qRT-PCR] and samples were calibrated by Beta-actin
Results: Western blot analysis discovered high expression of TLR7 and MAPK in HIOEC cell lines. TLR7 was over-expressed in HIOEC cell line. Imiquimod-induced expression of interleukin [IL]-6, IL-8, and vascular endothelial growth factor [VEGF] was inhibited by TLR7 siRNA in HIOEC cells as determined by reverse transcription polymerase chain reaction [RT-PCR]. Mean fluorescence intensity of nuclear p38 expression was determined in HIOEC cell lines [p< 0.05]. RT-PCR analysis of IL-6, IL-8, and VEGF mRNA expression in HIOEC cells stimulated with imiquimod [1 Mug/ml] for indicated time points
Conclusion: TLR7 is functionally over-expressed in HIOEC cell line of oral squamous cell carcinoma and development of resistance to cisplatin in human oral squamous cell carcinoma might occur through the mechanism involving activation of TLR7 and its dependent signaling pathway
RESUMEN
To study the effect of erythropoietin [EPO] treatment on renal and lung injury following renal ischemia/re-perfusion [I/R]. Thirty male Wistar rats were assigned to three groups of 10 rats each. The first group was sham-operated, the second was subjected to renal I/R [30 min of ischemia followed by 24 h of reperfusion]. The third group was subjected to renal I/R and treated with EPO in two doses: the first dose 1 h prior to ischemia [1,000 U/kg] and the second dose 6 h after ischemia [1,000 U/kg]. The renal and lung tissue injury index, tissue serum blood urea nitrogen and creatinine [Cr] were higher in the renal I/R group compared to the renal I/R + EPO group; the difference was statistically significant [p < 0.05]. Kidney and lung tissue glutathione peroxidase and superoxide dismutase levels were higher in the renal I/R + EPO group than the renal I/R group; the difference was also statistically significant [p < 0.05]. The data showed that EPO pretreatment could be effective in reducing renal and lung injury following renal I/R and could improve the cellular antioxidant defense system. Hence EPO pretreatment may be effective for attenuating renal and lung injury after renal I/R-induced injury during surgical procedures, hypotension, renal transplantation and other conditions inducing renal I/R