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J Genet ; 2009 Apr; 88(1): 25-31
Artículo en Inglés | IMSEAR | ID: sea-114501

RESUMEN

The mating system and seed variation of Acacia hybrid (A. mangium x A. auriculiformis) were studied using allozymes and random amplified polymorphic DNA (RAPD) markers, respectively. Multi-locus outcrossing rate estimations indicated that the hybrid was predominantly outcrossed (mean+/- s.e. tm = 0.86+/-0.01). Seed variation was investigated using 35 polymorphic RAPD fragments. An analysis of molecular variance (AMOVA) revealed the highest genetic variation among seeds within a pod (66%-70%), followed by among pods within inflorescence (29%-37%), and the least variation among inflorescences within tree (1%). In addition, two to four RAPD profiles could be detected among seeds within pod. Therefore, the results suggest that a maximum of four seeds per pod could be sampled for the establishment of a mapping population for further studies.


Asunto(s)
Acacia/genética , Cruzamientos Genéticos , ADN de Plantas/química , Genes de Plantas , Variación Genética , Genética de Población , Isoenzimas/genética , Semillas/genética
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