RESUMEN
A simple, sensitive and precise high performance liquid chromatographic method for the analysis of Pantoprazole sodium and lansoprazole has been developed, validated and used for the determination of compounds in commercial pharmaceutical products. The compounds were well separated an isocratically on a C18 column [Use Inertsil C18, 5m , 150 mm x 4.6 mm] utilizing a mobile phase consisting of acetonitrile: phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 1.0 ml/min with UV detection at 230 nm. The retention time of Pantoprazole sodium and lansoprazole was found to be 2.017 min and 2.538. The procedure was validated for linearity (Correlation coefficient = 0.999). The study showed that reversed-phase liquid chromatography is sensitive and selective for the determination of Pantoprazole sodium and lansoprazole using single mobile phase.
RESUMEN
A simple, selective, accurate reverse phase-high Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for the analysis of Sildenafil Citrate in pharmaceutical formulations. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5m , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (70:30, v/v, pH 7.0) at a flow rate of 0.8 ml/m with UV detection at 228 nm. The retention time was 4.087. The method is accurate (99.15-101.85%), precise (intraday variation 0.13-1.56% and inter-day variation 0.30-1.60%) and linear within range 0.1- 30μg/ml (R2=0.999) concentration and was successfully used in monitoring left over drug. The detection limit of sildenafil citrate at a signal-to-noise ratio of 3 was 1.70ng/ml in formulations while quantification limit in drug was 5.40 ng/ml. The proposed method is applicable to stability studies and routine analysis of sildenafil citrate in pharmaceutical formulations.