Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
J Biosci ; 2007 Mar; 32(2): 251-60
Artículo en Inglés | IMSEAR | ID: sea-110868

RESUMEN

Entamoeba histolytica contains a novel calcium-binding protein like calmodulin,which was discovered earlier,and we have reported the presence of its homologue(s)and a dependent protein kinase in plants.To understand the functions of these in plants,a cDNA encoding a calcium-binding protein isolated from Entamoeba histolytica (EhCaBP)was cloned into vector pBI121 in antisense orientation and transgenic tobacco plants were raised.These plants showed variation in several phenotypic characters,of which two distinct features,more greenness and leaf thickness,were inherited in subsequent generations.The increase in the level of total chlorophyll in different plants ranged from 60% to 70%.There was no major change in chloroplast structure and in the protein level of D1,D2,LHCP and RuBP carboxylase.These morphological changes were not seen in antisense calmodulin transgenic tobacco plants,nor was the calmodulin level altered in EhCaBP antisense plants.


Asunto(s)
Animales , Northern Blotting , Southern Blotting , Western Blotting , Proteínas de Unión al Calcio/genética , Clorofila/biosíntesis , Citocininas/metabolismo , ADN sin Sentido/metabolismo , Entamoeba histolytica/genética , Ensayo de Inmunoadsorción Enzimática , Vectores Genéticos/genética , Microscopía Electrónica de Transmisión , Fenotipo , Hojas de la Planta/metabolismo , Plantas Modificadas Genéticamente/genética , Poliaminas/metabolismo , Nicotiana/anatomía & histología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA